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Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750333

Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] - BSA and Azide free

Application

  • WB

  • IP

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD71 aa 22-60 / 760.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP, FC

Molecular Weight

Predicted band size: 85 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela.

Conjugation

unconjugated

Clone Number

JF0956

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:10,000

  • FC

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.

Background References

1. Xiong L et al. Nutrition impact on ILC3 maintenance and function centers on a cell-intrinsic CD71-iron axis. Nat Immunol. 2023 Oct;

2. Chiappelli F. CD71: Role in permafrost immunity. Bioinformation. 2024 Mar

Sequence Similarity

Belongs to the peptidase M28 family. M28B subfamily.

Post-translational Modification

N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.; Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

Subcellular Location

Cell membrane, Melanosome, Secreted.

UNIPROT

SwissProt: P02786 Human

SwissProt: Q62351 Mouse

SwissProt: Q99376 Rat

Synonyms

CD 71 antibody

CD71 antibody

CD71 antigen antibody

IMD46 antibody

OTTHUMP00000208523 antibody

OTTHUMP00000208524 antibody

OTTHUMP00000208525 antibody

p90 antibody

sTfR antibody

T9 antibody

Expand

Images

  • Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: SW480 cell lysate<br />Lane 4: U-87 MG cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: Mouse spleen tissue lysate<br />Lane 7: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 5 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (HA750333) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: SW480 cell lysate
    Lane 4: U-87 MG cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: Mouse spleen tissue lysate
    Lane 7: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa

    Exposure time: 5 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750333) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si Transferrin Receptor cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 1 minute 20 seconds;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (HA750333) at 1/1,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si Transferrin Receptor cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa

    Exposure time: 1 minute 20 seconds;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750333) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1 WT cell lysate<br />Lane 2: HAP1 Transferrin Receptor KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 1 minute 48 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (HA750333) at 1/5,000 dilution.

    Lane 1: HAP1 WT cell lysate
    Lane 2: HAP1 Transferrin Receptor KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 90 kDa

    Exposure time: 1 minute 48 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750333) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA750333" style="font-weight: bold;text-decoration: underline;">HA750333</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71).

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA750333, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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