VAMP8 Recombinant Rabbit Monoclonal Antibody [JF0963] - BSA and Azide free
Catalog# HA750334
VAMP8 Recombinant Rabbit Monoclonal Antibody [JF0963] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC(Intra)
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
VAMP8 Recombinant Rabbit Monoclonal Antibody [JF0963] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide wiithin Human VAMP8 aa1-29 / 100.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC(Intra), IP
Target Molecular Weight
Predicted band size: 11 kDa
Positive Control
Hela cell lysate, mouse kidney tissue lysate, U937 cell lysates, SW480, 293T, Hela, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
JF0963
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene.Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.Phosphorylation of VAMP8 inside the conserved SNARE-domain can suppress vesicle fusion.
Background References
1. Xie X et al. Deep vein thrombosis is accurately predicted by comprehensive analysis of the levels of microRNA-96 and plasma D-dimer. Exp Ther Med 12:1896-1900 (2016).
2. Pirooz SD et al. UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs. Proc Natl Acad Sci U S A 111:2716-21 (2014).
Sequence Similarity
Belongs to the synaptobrevin family.
Tissue Specificity
Platelets.
Subcellular Location
Lysosome membrane, Early endosome membrane, Late endosome membrane, Cell membrane.
Synonyms
EDB antibody
Endobrevin antibody
VAMP 8 antibody
VAMP-8 antibody
VAMP8 antibody
VAMP8_HUMAN antibody
Vesicle associated membrane protein 8 antibody
Vesicle-associated membrane protein 8 antibody
Images
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This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Western blot analysis of VAMP8 on HL-60 cell/tissue lysates with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 15 kDa
Exposure time: 1 minutes 55 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-10) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HL-60 cells labeling VAMP8 with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-10) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-10) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-10) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HL-60 cells labeling VAMP8.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-10, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
This data was developed using ET1702-10, the same antibody clone in a different buffer formulation.
Western blot analysis of VAMP8 on different lysates with Rabbit anti-VAMP8 antibody (ET1702-10) at 1/2,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 41 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1702-10, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 11 kDa
Observed band size: 15 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"