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LYVE1 Recombinant Rabbit Monoclonal Antibody [JF0979] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750343

LYVE1 Recombinant Rabbit Monoclonal Antibody [JF0979] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

LYVE1 Recombinant Rabbit Monoclonal Antibody [JF0979] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human LYVE1 aa 36-72 / 322.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 35 kDa

Positive Control

MCF-7 cell lysate, SW480 cell lysate, A431 cell lysate, HUVEC cell lysate, A431, HUVEC, SW480.

Conjugation

unconjugated

Clone Number

JF0979

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1), also known as extracellular link domain containing 1 (XLKD1) is a Link domain-containing hyaladherin, a protein capable of binding to hyaluronic acid (HA), homologous to CD44, the main HA receptor. In humans it is encoded by the LYVE1 gene. LYVE1 is a type I integral membrane glycoprotein. It acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. LYVE-1 is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, allowing for the isolation of these cells for experimental purposes. The physiological role for this receptor is still the subject of debate, but evolutionary conservation suggests an important role. Expression of LYVE1 not restricted to lymph vessels but is also observed in normal liver blood sinusoids, and embryonic blood vessels.

Background References

1. Yu X et al. Septal LYVE1(+) macrophages control adipocyte stem cell adipogenic potential. Science. 2025 Aug

2. Siret C et al. Deciphering the heterogeneity of the Lyve1(+) perivascular macrophages in the mouse brain. Nat Commun. 2022 Nov

Tissue Specificity

Mainly expressed in endothelial cells lining lymphatic vessels.

Post-translational Modification

O-glycosylated.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q9Y5Y7 Human

Synonyms

Cell surface retention sequence-binding protein 1 antibody

CRSBP 1 antibody

CRSBP-1 antibody

CRSBP1 antibody

extracellular link domain containing 1 antibody

extracellular link domain-containing 1 antibody

Extracellular link domain-containing protein 1 antibody

HAR antibody

Hyaluronic acid receptor antibody

Lymphatic endothelium specific hyaluronan receptor antibody

Expand

Images

  • Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (<a href="/products/HA750343" style="font-weight: bold;text-decoration: underline;">HA750343</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF-7 cell lysate<br />Lane 2: SW480 cell lysate<br />Lane 3: A431 cell lysate<br />Lane 4: HUVEC cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 2 minutes;<br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750343" style="font-weight: bold;text-decoration: underline;">HA750343</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (HA750343) at 1/2,000 dilution.

    Lane 1: MCF-7 cell lysate
    Lane 2: SW480 cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HUVEC cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 2 minutes;
    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750343) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (<a href="/products/HA750343" style="font-weight: bold;text-decoration: underline;">HA750343</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si LYVE1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-29" style="font-weight: bold;text-decoration: underline;">ET1702-29</a>, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (HA750343) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si LYVE1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"