KLF4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JF98-08] (HA750356)
Catalog# HA750356
Rabbit Monoclonal to KLF4
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KLF4 on different lysates with Rabbit anti-KLF4 antibody (HA750356) at 1/2,000 dilution.
Lane 1: 293 cell lysate
Lane 2: NCCIT cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750356) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: 293 cell lysate
Lane 2: NCCIT cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750356) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.