KLF4 Recombinant Rabbit Monoclonal Antibody [JF98-08] - BSA and Azide free
Catalog# HA750356
KLF4 Recombinant Rabbit Monoclonal Antibody [JF98-08] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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ET1702-71
含抗保成分
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unconjugated
Overview
Product Name
KLF4 Recombinant Rabbit Monoclonal Antibody [JF98-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human KLF4 aa 320-360.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
293 cell lysate, NCCIT cell lysate, C6 cell lysate, Hela, PC-3M, 293T, human stomach tissue, mouse stomach tissue, rat stomach tissue.
Conjugation
unconjugated
Clone Number
JF98-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF (6-8). EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arres.
Background References
1. Abu-Hassan DW et al. Induced pluripotent stem cells restore function in a human cell loss model of open-angle glaucoma. Stem Cells 33:751-61 (2015).
2. Jia D et al. -Catenin and NF-kB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Cell Death Differ 22:298-310 (2015).
Sequence Similarity
Belongs to the krueppel C2H2-type zinc-finger protein family.
Subcellular Location
Nucleus.
Synonyms
Endothelial Kruppel like zinc finger protein antibody
Epithelial zinc finger protein EZF antibody
EZF antibody
GKLF antibody
gut Kruppel-like factor antibody
Gut-enriched krueppel-like factor antibody
KLF antibody
KLF4 antibody
KLF4_HUMAN antibody
Krueppel-like factor 4 antibody
ExpandImages
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Western blot analysis of KLF4 on different lysates with Rabbit anti-KLF4 antibody (HA750356) at 1/2,000 dilution.
Lane 1: 293 cell lysate
Lane 2: NCCIT cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750356) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of KLF4 on C6 cell lysate with Rabbit anti-KLF4 antibody (HA750356) at 1/5,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55/62 kDa
Observed band size: 55/62 kDa
Exposure time: 4 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750356) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature. -
ICC staining of KLF4 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750356, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of KLF4 in PC-3M cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750356, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of KLF4 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750356, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Stomach
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA750356, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Stomach
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA750356, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Stomach
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA750356, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"