CHOP/DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31] - BSA and Azide free
Catalog# HA750369
CHOP/DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31] - BSA and Azide free
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WB
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FC(Intra)
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CHOP/DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human DDIT3 aa 135-169 / 169.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC(Intra)
Target Molecular Weight
Predicted band size: 19 kDa
Positive Control
SW480 cell lysate, HeLa cell lysate, Hela.
Conjugation
unconjugated
Clone Number
JM10-31
Reactivity Data
| WB | FC(Intra) | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotic transcription factor that is encoded by the DDIT3 gene. It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis and has an important role in the cell's stress response. The regulation of CHOP expression plays an important role in metabolic diseases and in some cancers through its function in mediating apoptosis. The regulation of CHOP expression could be a potential approach to affecting cancer cells through the induction of apoptosis. In the intestinal epithelium, CHOP has been demonstrated to be downregulated under inflammatory conditions (in inflammatory bowel diseases and experimental models of colitis). In this context, CHOP seems to rather regulate the cell cycle than apoptotic processes. Mutations or fusions of CHOP (e.g. with FUS to form FUS-CHOP) can cause Myxoid liposarcoma.
Background References
1. Sun XY et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress-induced apoptosis. Mol Med Rep 13:661-8 (2016).
2. Greenwood M et al. Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus. PLoS One 10:e0124956 (2015).
Sequence Similarity
Belongs to the bZIP family.
Post-translational Modification
Ubiquitinated, leading to its degradation by the proteasome.; Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
C/EBP homologous protein antibody
C/EBP Homology Protein antibody
C/EBP zeta antibody
C/EBP-homologous protein 10 antibody
C/EBP-homologous protein antibody
CCAAT/enhancer binding protein homologous protein antibody
CEBPZ antibody
CHOP 10 antibody
CHOP antibody
CHOP-10 antibody
ExpandImages
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This data was developed using ET1703-05, the same antibody clone in a different buffer formulation.
Western blot analysis of CHOP/DDIT3 on different lysates with Rabbit anti-CHOP/DDIT3 antibody (ET1703-05) at 1/5,000 dilution.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 27 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-05) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using ET1703-05, the same antibody clone in a different buffer formulation.
Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (ET1703-05) at 1/1,000 dilution.
Lane 1: SW480-si NT cell lysate
Lane 2: SW480-si DDIT3 cell lysate
Lysates/proteins at 3 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 25 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
ET1703-05 was shown to specifically react with DDIT3 in SW480-si NT cells. Weakened band was observed when SW480-si DDIT3 sample was tested. SW480-si NT and SW480-si DDIT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-05, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1703-05, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of CHOP/DDIT3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-05, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
This data was developed using ET1703-05, the same antibody clone in a different buffer formulation.
Western blot analysis of CHOP/DDIT3 on different lysates with Rabbit anti-CHOP/DDIT3 antibody (ET1703-05) at 1/5,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: C2C12 treatment with 300 nM thapsigargin for 18h cell lysate
Lane 3: C6 cell lysate
Lane 4: C6 treatment with 300 nM thapsigargin for 18h cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1703-05, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 19 kDa
Observed band size: 27 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CHOP/DDIT3 Recombinant Rabbit Monoclonal Antibody [PSH07-65]
Application: WB,IF-Cell,FC(Intra)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CHOP/DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31]
Application: WB,FC(Intra)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
