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ERCC1 Recombinant Rabbit Monoclonal Antibody [JM10-07] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750374

ERCC1 Recombinant Rabbit Monoclonal Antibody [JM10-07] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Predicted reactivity

  • Mouse

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

ERCC1 Recombinant Rabbit Monoclonal Antibody [JM10-07] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ERCC1 aa 1-220 / 297.

Species Reactivity

Human (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 33 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Raji cell lysate, Hela, A549, HepG2, PANC-1, 293T, human kidney tissue.

Conjugation

unconjugated

Clone Number

JM10-07

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50

  • IF-Tissue

  • 1:50

  • IHC-P

  • 1:50-1:100

Target

Function

DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene. Together with ERCC4, ERCC1 forms the ERCC1-XPF enzyme complex that participates in DNA repair and DNA recombination. Cells with disabling mutations in ERCC1 are more sensitive than normal to particular DNA damaging agents, including ultraviolet (UV) radiation and to chemicals that cause crosslinking between DNA strands. Genetically engineered mice with disabling mutations in ERCC1 have defects in DNA repair, accompanied by metabolic stress-induced changes in physiology that result in premature aging. Complete deletion of ERCC1 is incompatible with viability of mice, and no human individuals have been found with complete (homozygous) deletion of ERCC1. Rare individuals in the human population harbor inherited mutations that impair the function of ERCC1. When the normal genes are absent, these mutations can lead to human syndromes, including Cockayne syndrome (CS) and COFS.

Background References

1. Millis SZ et al. Molecular Profiling of Refractory Adrenocortical Cancers and Predictive Biomarkers to Therapy. Biomark Cancer 7:69-76 (2015).

2. Muallem MZ et al. ERCC1 expression as a predictor of resistance to platinum-based chemotherapy in primary ovarian cancer. Anticancer Res 34:393-9 (2014).

Sequence Similarity

Belongs to the ERCC1/RAD10/SWI10 family.

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P07992 Human

SwissProt: P07903 Mouse

Synonyms

COFS 4 antibody

COFS4 antibody

DNA excision repair protein ERCC 1 antibody

DNA excision repair protein ERCC-1 antibody

DNA excision repair protein ERCC1 antibody

ERCC 1 antibody

ERCC1 antibody

ERCC1_HUMAN antibody

Excision repair cross complementation group 1 antibody

Excision repair cross complementing 1 antibody

Expand

Images

  • Western blot analysis of ERCC1 on different lysates with Rabbit anti-ERCC1 antibody (<a href="/products/HA750374" style="font-weight: bold;text-decoration: underline;">HA750374</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: SK-Br-3 cell lysate<br />Lane 5: Raji cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750374" style="font-weight: bold;text-decoration: underline;">HA750374</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ERCC1 on different lysates with Rabbit anti-ERCC1 antibody (HA750374) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: SK-Br-3 cell lysate
    Lane 5: Raji cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 38 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750374) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"