CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] - BSA and Azide free

Function

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Background References

1. Vojta A et al. Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res N/A:N/A (2016).

2. Cinesi C et al. Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Nat Commun 7:13272 (2016).

Sequence Similarity

Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily.

UNIPROT

SwissProt: Q99ZW2 StreptococcusPyogenes

Synonyms

Cas9 antibody

CRISPR-associated endonuclease Cas9/Csn1 antibody

CRISPR-Cas9/Csn1 antibody

csn1 antibody

SpyCas9 antibody