MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72] - BSA and Azide free
Catalog# HA750395
MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72] - BSA and Azide free
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WB
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IP
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FC
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Human
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unconjugated
Overview
Product Name
MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MyoD1 aa 36-80 / 320.
Species Reactivity
Human
Validated Applications
WB, IP, FC
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
Hela cell lysates, Hela.
Conjugation
unconjugated
Clone Number
JM10-72
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IP
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1:10-1:50
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FC
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1:50-1:100
Target
Function
MyoD, also known as myoblast determination protein 1, is a protein in animals that plays a major role in regulating muscle differentiation. MyoD, which was discovered in the laboratory of Harold M. Weintraub, belongs to a family of proteins known as myogenic regulatory factors (MRFs). These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation. Vertebrate MRF family members include MyoD1, Myf5, myogenin, and MRF4 (Myf6). In non-vertebrate animals, a single MyoD protein is typically found. MyoD is one of the earliest markers of myogenic commitment. MyoD is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression of MyoD is activated in response to exercise or muscle tissue damage. The effect of MyoD on satellite cells is dose-dependent; high MyoD expression represses cell renewal, promotes terminal differentiation and can induce apoptosis. Although MyoD marks myoblast commitment, muscle development is not dramatically ablated in mouse mutants lacking the MyoD gene. This is likely due to functional redundancy from Myf5 and/or Mrf4. Nevertheless, the combination of MyoD and Myf5 is vital to the success of myogenesis.
Background References
1. Ahmed AA et al. MYOD1 as a prognostic indicator in rhabdomyosarcoma. Pediatr Blood Cancer. 2021 Sep
2. Di Carlo D et al. Biological Role and Clinical Implications of MYOD1L122R Mutation in Rhabdomyosarcoma. Cancers (Basel). 2023 Mar
Post-translational Modification
Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.; Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).; Ubiquitinated on the N-terminus; which is required for proteasomal degradation.; Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
bHLHc1 antibody
Class C basic helix-loop-helix protein 1 antibody
MYF 3 antibody
Myf-3 antibody
MYF3 antibody
Myoblast determination protein 1 antibody
Myod 1 antibody
MYOD antibody
MYOD1 antibody
MYOD1_HUMAN antibody
ExpandImages
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Western blot analysis of MyoD1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750395, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of MyoD1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750395, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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