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MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750395

MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72] - BSA and Azide free

Application

  • WB

  • IP

  • FC

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MyoD1 aa 36-80 / 320.

Species Reactivity

Human

Validated Applications

WB, IP, FC

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Hela cell lysates, Hela.

Conjugation

unconjugated

Clone Number

JM10-72

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IP

  • 1:10-1:50

  • FC

  • 1:50-1:100

Target

Function

MyoD, also known as myoblast determination protein 1, is a protein in animals that plays a major role in regulating muscle differentiation. MyoD, which was discovered in the laboratory of Harold M. Weintraub, belongs to a family of proteins known as myogenic regulatory factors (MRFs). These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation. Vertebrate MRF family members include MyoD1, Myf5, myogenin, and MRF4 (Myf6). In non-vertebrate animals, a single MyoD protein is typically found. MyoD is one of the earliest markers of myogenic commitment. MyoD is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression of MyoD is activated in response to exercise or muscle tissue damage. The effect of MyoD on satellite cells is dose-dependent; high MyoD expression represses cell renewal, promotes terminal differentiation and can induce apoptosis. Although MyoD marks myoblast commitment, muscle development is not dramatically ablated in mouse mutants lacking the MyoD gene. This is likely due to functional redundancy from Myf5 and/or Mrf4. Nevertheless, the combination of MyoD and Myf5 is vital to the success of myogenesis.

Background References

1. Ahmed AA et al. MYOD1 as a prognostic indicator in rhabdomyosarcoma. Pediatr Blood Cancer. 2021 Sep

2. Di Carlo D et al. Biological Role and Clinical Implications of MYOD1L122R Mutation in Rhabdomyosarcoma. Cancers (Basel). 2023 Mar

Post-translational Modification

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.; Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).; Ubiquitinated on the N-terminus; which is required for proteasomal degradation.; Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P15172 Human

Synonyms

bHLHc1 antibody

Class C basic helix-loop-helix protein 1 antibody

MYF 3 antibody

Myf-3 antibody

MYF3 antibody

Myoblast determination protein 1 antibody

Myod 1 antibody

MYOD antibody

MYOD1 antibody

MYOD1_HUMAN antibody

Expand

Images

  • Western blot analysis of MyoD1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA750395" style="font-weight: bold;text-decoration: underline;">HA750395</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MyoD1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750395, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of MyoD1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA750395" style="font-weight: bold;text-decoration: underline;">HA750395</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of MyoD1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750395, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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