ADFP / PLIN2 Recombinant Rabbit Monoclonal Antibody [JA31-81] - BSA and Azide free
Catalog# HA750407
ADFP / PLIN2 Recombinant Rabbit Monoclonal Antibody [JA31-81] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ADFP / PLIN2 Recombinant Rabbit Monoclonal Antibody [JA31-81] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ADFP aa 18-67 / 437.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Tissue, IHC-P
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
HepG2 cell lysate, JAR cell lysate, Human liver tissue lysate, human liver tissue, human liver carcinoma tissue, human stomach tissue.
Conjugation
unconjugated
Clone Number
JA31-81
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Background References
1. Cunnick JM et al. Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland. Oncogene 0:N/A (2014).
2. Tinelli E et al. Muscle-specific function of the centronuclear myopathy and Charcot-Marie-Tooth neuropathy-associated dynamin 2 is required for proper lipid metabolism, mitochondria, muscle fibers, neuromuscular junctions and peripheral nerves. Hum Mol Genet 22:4417-29 (2013).
Sequence Similarity
Belongs to the perilipin family.
Tissue Specificity
Milk lipid globules.
Post-translational Modification
Acylated; primarily with C14, C16 and C18 fatty acids.
Subcellular Location
Membrane.
Synonyms
ADFP antibody
Adipophilin antibody
Adipose differentiation related protein antibody
Adipose differentiation-related protein antibody
ADRP antibody
MGC10598 antibody
Perilipin-2 antibody
Perilipin 2 antibody
PLIN2 antibody
PLIN2_HUMAN antibody
Images
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This data was developed using ET1704-17, the same antibody clone in a different buffer formulation.
Western blot analysis of ADFP / PLIN2 on different lysates with Rabbit anti-ADFP / PLIN2 antibody (ET1704-17) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: JAR cell lysate
Lane 3: Human liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using ET1704-17, the same antibody clone in a different buffer formulation.
Western blot analysis of ADFP / PLIN2 on different lysates with Rabbit anti-ADFP / PLIN2 antibody (ET1704-17) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ADFP / PLIN2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-17) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1704-17, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ADFP / PLIN2 antibody (ET1704-17) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-17) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1704-17, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-ADFP / PLIN2 antibody (ET1704-17) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-17) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1704-17, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ADFP / PLIN2 antibody (ET1704-17) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-17) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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