CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] - BSA and Azide free
Catalog# HA750422
CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] - BSA and Azide free
-
WB
-
Human
-
unconjugated
Overview
Product Name
CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CXCR3 aa 278-298 (Extracellular)/368.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, PANC-1 cell lysate, K-562 cell lysate, HT-29 cell lysate, Human lung tissue lysate.
Conjugation
unconjugated
Clone Number
JA61-33
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
Target
Function
Chemokine receptor CXCR3 is a Gαi protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. CXCR3 is able to regulate leukocyte trafficking. Binding of chemokines to CXCR3 induces various cellular responses, most notably integrin activation, cytoskeletal changes and chemotactic migration. CXCR3-ligand interaction attracts Th1 cells and promotes Th1 cell maturation. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. A hallmark of CXCR3 is its prominent expression in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, CXCL9, CXCL10 and CXCL11 are commonly produced by local cells in inflammatory lesion, suggesting that CXCR3 and its chemokines participate in the recruitment of inflammatory cells. Additionally, CXCR3 has been implicated in wound healing.
Background References
1. Tanaka A et al. Th2 and regulatory immune reactions contribute to IgG4 production and the initiation of Mikulicz disease. Arthritis Rheum 64:254-63 (2012).
2. Song CH et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).
Post-translational Modification
Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.; N-glycosylated.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
C-X-C chemokine receptor type 3 antibody
CD 183 antibody
CD182 antibody
CD183 antibody
Chemokine (C X C motif) receptor 3 antibody
Chemokine (C X C) receptor 3 antibody
Chemokine CXC Motif Receptor 3 antibody
CKR L2 antibody
CKR-L2 antibody
CKRL2 antibody
ExpandImages
-
Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (HA750422) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: K-562 cell lysate
Lane 5: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750422) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CXCR3 on different lysates with Rabbit anti-CXCR3 antibody (HA750422) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (10 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time: 57 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750422) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
