SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44] - BSA and Azide free
Catalog# HA750449
SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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IF-Cell
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FC
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SATB2 238-287 / 733.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue, IF-Cell, FC
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
THP-1 cell lysate, Saos-2 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human colon carcinoma tissue, mouse brain tissue, rat brain tissue, mouse colon tissue, rat colon tissue, rat large intestine tissue, Saos-2.
Conjugation
unconjugated
Clone Number
JM52-44
Reactivity Data
| WB | IHC-P | IHC-Fr | IF-Tissue | IF-Cell | FC | |
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| Human |
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| Mouse |
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| Rat |
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| Cynomolgus Monkey |
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| Pig |
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.
Background References
1. Gu W et al. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell. 2022 Jan
2. Roy SK et al. SATB2 is a novel biomarker and therapeutic target for cancer. J Cell Mol Med. 2020 Oct
Sequence Similarity
Belongs to the CUT homeobox family.
Tissue Specificity
High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
Post-translational Modification
Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
Subcellular Location
Nucleus matrix.
Synonyms
DNA binding protein SATB2 antibody
DNA-binding protein SATB2 antibody
FLJ21474 antibody
FLJ32076 antibody
GLSS antibody
KIAA1034 antibody
MGC119474 antibody
MGC119477 antibody
SATB family member 2 antibody
SATB homeobox 2 antibody
ExpandImages
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This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex (P7)
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Application: IF-Tissue
Species: Mouse
Site: brain
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Western blot analysis of SATB2 on different lysates with Rabbit anti-SATB2 antibody (ET1705-95) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate (15 µg/Lane)
Lane 2: Saos-2 cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-95) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SATB2 antibody (ET1705-95) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-95) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SATB2 antibody (ET1705-95) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-95) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SATB2 antibody (ET1705-95) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-95) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SATB2 antibody (ET1705-95) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-95) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SATB2 antibody (ET1705-95) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-95) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-SATB2 antibody (ET1705-95) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-95) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-95, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Saos-2 cells labeling SATB2 with Rabbit anti-SATB2 antibody (ET1705-95) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SATB2 antibody (ET1705-95) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"