HPRT Recombinant Rabbit Monoclonal Antibody [JU03-26] - BSA and Azide free
Catalog# HA750452
HPRT Recombinant Rabbit Monoclonal Antibody [JU03-26] - BSA and Azide free
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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Zebrafish
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unconjugated
Overview
Product Name
HPRT Recombinant Rabbit Monoclonal Antibody [JU03-26] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HPRT aa 169-218 / 218.
Species Reactivity
Human, Mouse, Rat, Zebrafish
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 25 kDa
Positive Control
HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, zebrafish tissue lysates.
Conjugation
unconjugated
Clone Number
JU03-26
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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| Zebrafish |
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.
Background References
1. Fernández-Mosquera L et al. Acute and chronic mitochondrial respiratory chain deficiency differentially regulate lysosomal biogenesis. Sci Rep 7:45076 (2017).
2. Angelika Ihle M et al. HR23b expression is a potential predictive biomarker for HDAC inhibitor treatment in mesenchymal tumours and is associated with response to vorinostat. J Pathol Clin Res 2:59-71 (2016).
Sequence Similarity
Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Subcellular Location
Cytoplasm.
Synonyms
HGPRT antibody
HGPRTase antibody
HPRT 1 antibody
HPRT_HUMAN antibody
HPRT1 antibody
Hypoxanthine guanine phosphoribosyltransferase antibody
Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody
Hypoxanthine phosphoribosyltransferase 1 antibody
Hypoxanthine-guanine phosphoribosyltransferase antibody
Images
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This data was developed using ET1706-08, the same antibody clone in a different buffer formulation.
Western blot analysis of HPRT on zebrafish tissue lysates with Rabbit anti-HPRT antibody (ET1706-08) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 2 minutes 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-08) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1706-08, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon cancer
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1706-08, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using ET1706-08, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1706-08, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using ET1706-08, the same antibody clone in a different buffer formulation.
Western blot analysis of HPRT on different lysates with Rabbit anti-HPRT antibody (ET1706-08) at 1/2,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 4: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 8 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1706-08, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 25 kDa
Observed band size: 25 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"