SCGB1A1 Recombinant Rabbit Monoclonal Antibody [JU34-03] - BSA and Azide free
Catalog# HA750458
SCGB1A1 Recombinant Rabbit Monoclonal Antibody [JU34-03] - BSA and Azide free
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WB
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IF-Cell
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FC
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Human
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Mouse
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ET7106-71
含抗保成分
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
SCGB1A1 Recombinant Rabbit Monoclonal Antibody [JU34-03] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SCGB1A1 aa 42-91 / 91.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 10 kDa
Positive Control
Mouse lung tissue lysate, human lung tissue lysate, A549 cell lysate, A431, A549, HUVEC.
Conjugation
unconjugated
Clone Number
JU34-03
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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FC
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1:50-1:100
Target
Function
SCGB1A1 also named CC10. Clara cell 10 (CC10) protein, a homologue of rabbit uteroglobin, is a phospholipase A2 inhibitor. CC10 is regulated by AP-1, octamer, and hepatocyte nuclear factor-3 (HNF-3) family transcription factors. CC10 expression changes in relation to the ovarian menstrual cycle, and expression in human endometrium may be stimulated by progesterone, suggesting that CC10 may regulate eicosanoid levels in the human uterus. CC10 is expressed in nonciliated airway epithelial cells in the lung and in urogenital secretions. CC10 is involved in modulating inflammation in airway passages and may play a role in asthma. Overexpression of CC10 in the non-small cell lung cancer cell line A549 was shown to result in the near absence of MMP-2 and MMP-9 matrix metalloproteinases and a reduction in invasiveness, indicating that loss of CC10 may contribute to carcinogenesis.
Background References
1. Chen X et al. SCGB1A1 as a Key Regulator of Splenic Immune Dysfunction in COPD: Insights From a Murine Model. Int J Chron Obstruct Pulmon Dis. 2025 Mar
2. Wang J et al. SCGB1A1 as a novel biomarker and promising therapeutic target for the management of HNSCC. Oncol Lett. 2024 Sep
Sequence Similarity
Belongs to the secretoglobin family.
Tissue Specificity
Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
Subcellular Location
Secreted.
Synonyms
Blastokinin antibody
CC10 antibody
CC16 antibody
CCPBP antibody
CCSP antibody
Clara cell phospholipid binding protein antibody
Clara cell phospholipid-binding protein antibody
Clara cell specific 10 kD protein antibody
Clara cells 10 kDa secretory protein antibody
OTTHUMP00000236107 antibody
ExpandImages
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Western blot analysis of SCGB1A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750458, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse lung tissue lysate
Lane 2: human lung tissue lysate
Lane 3: A549 cell lysate -
ICC staining of SCGB1A1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750458, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of SCGB1A1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750458, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of SCGB1A1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750458, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of SCGB1A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750458, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"