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Ctip1 Recombinant Rabbit Monoclonal Antibody [JB35-68] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750461

Ctip1 Recombinant Rabbit Monoclonal Antibody [JB35-68] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Ctip1 Recombinant Rabbit Monoclonal Antibody [JB35-68] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Ctip1 aa 381-609 / 835.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC, IF-Cell, IF-Tissue

Molecular Weight

Predicted band size: 91 kDa

Positive Control

Jurkat cell lysates, Daudi cell lysate, Raji cell lysate, Jurkat, mouse brain tissue, mouse spleen tissue.

Conjugation

unconjugated

Clone Number

JB35-68

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50

  • FC

  • 1:1,000

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:50-1:200

Target

Function

The BCL11A gene encodes for a regulatory C2H2 type zinc-finger protein, that can bind to the DNA. Five alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been reported. The protein associates with the SWI/SNF complex, that regulates gene expression via chromatin remodelling. BCL11A is highly expressed in several hematopoietic lineages, and plays a role in the switch from γ- to β-globin expression during the fetal to adult erythropoiesis transition. Furthermore, BCL11A is expressed in the brain, where it forms a protein complex with CASK to regulate axon outgrowth and branching. In the neocortex, BCL11A binds to the TBR1 regulatory region and inhibits the expression of TBR1.

Background References

1. Bauer D E et al. Hemoglobin switching\'s surprise: the versatile transcription factor BCL11A is a master repressor of fetal hemoglobin. Curr Opin Genet Dev 33:62-70 (2015).

2. Dias C et al. BCL11A haploinsufficiency causes an intellectual disability syndrome and dysregulates transcription. Am J Hum Genet 99:253-274 (2016).

Tissue Specificity

Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.

Post-translational Modification

Sumoylated with SUMO1.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: Q9H165 Human

SwissProt: Q9QYE3 Mouse

Synonyms

2810047E18Rik antibody

B cell CLL/lymphoma 11A (zinc finger protein) antibody

B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody

B-cell CLL/lymphoma 11A antibody

B-cell lymphoma/leukemia 11A antibody

BC11A_HUMAN antibody

BCL-11A antibody

BCL11A antibody

BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody

BCL11A L antibody

Expand

Images

  • Western blot analysis of Ctip1 on Jurkat cell lysates with Rabbit anti-Ctip1 antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 91 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ctip1 on Jurkat cell lysates with Rabbit anti-Ctip1 antibody (HA750461) at 1/5,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 91 kDa
    Observed band size: 120 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750461) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Ctip1 on different lysates with Rabbit anti-Ctip1 antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate<br />Lane 2: Raji cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 91 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ctip1 on different lysates with Rabbit anti-Ctip1 antibody (HA750461) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate
    Lane 2: Raji cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 91 kDa
    Observed band size: 120 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750461) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Jurkat cells labeling Ctip1 with Rabbit anti-Ctip1 antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ctip1 antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Jurkat cells labeling Ctip1 with Rabbit anti-Ctip1 antibody (HA750461) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ctip1 antibody (HA750461) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Ctip1 antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Ctip1 antibody (HA750461) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750461) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ctip1 antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ctip1 antibody (HA750461) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750461) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Jurkat cells labeling Ctip1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA750461" style="font-weight: bold;text-decoration: underline;">HA750461</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling Ctip1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA750461, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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