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Villin1 Recombinant Rabbit Monoclonal Antibody [A2] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750477

Villin1 Recombinant Rabbit Monoclonal Antibody [A2] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Villin1 Recombinant Rabbit Monoclonal Antibody [A2] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Villin1 aa 600-827.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 93 kDa

Positive Control

HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, human kidney tissue, human small intestine tissue.

Conjugation

unconjugated

Clone Number

A2

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB
human
Tested Tested
mouse
Not Recommended
rat
Not Recommended

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

Background References

1. Nasser MET. et. al. Value of Plasmatic Villin-1 in the Prediction of Early Graft Dysfunction in Kidney Transplant Recipients: Diagnostic Accuracy Test. Exp Clin Transplant. 2021 Feb

2. Roy S. et. al. Villin-1 and Gelsolin Regulate Changes in Actin Dynamics That Affect Cell Survival Signaling Pathways and Intestinal Inflammation. Gastroenterology. 2018 Apr

Subcellular Location

Cytoskeleton, lamellipodium, ruffle, microvillus, filopodium tip, filopodium.

UNIPROT

SwissProt: P09327 Human

Synonyms

D2S1471 antibody

OTTHUMP00000164145 antibody

VIL antibody

VIL1 antibody

VILI_HUMAN antibody

Villin 1 antibody

Villin-1 antibody

Villin1 antibody

Images

  • This data was developed using <a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (<a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>) at 1/10,000 dilution.<br /><br />Lane 1: HT-29 (Human colorectal adenocarcinoma cell) cell lysate<br />Lane 2: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate<br />Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 17 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 93 kDa<br />Observed band size: 93 kDa

    This data was developed using HA721134, the same antibody clone in a different buffer formulation.
    Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (HA721134) at 1/10,000 dilution.

    Lane 1: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 2: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 17 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721134, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 93 kDa
    Observed band size: 93 kDa

  • This data was developed using <a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (<a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721134, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (HA721134) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721134) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Villin1 antibody (<a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721134" style="font-weight: bold;text-decoration: underline;">HA721134</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721134, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Villin1 antibody (HA721134) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721134) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"