RICTOR Recombinant Rabbit Monoclonal Antibody [1D5] - BSA and Azide free
Catalog# HA750483
RICTOR Recombinant Rabbit Monoclonal Antibody [1D5] - BSA and Azide free
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WB
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IF-Cell
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FC
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Human
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HA721141
含抗保成分
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unconjugated
Overview
Product Name
RICTOR Recombinant Rabbit Monoclonal Antibody [1D5] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RICTOR aa 1459-1708 / 1708.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 192 kDa
Positive Control
A549 cell lysate, SW480 cell lysate, A431, Hela.
Conjugation
unconjugated
Clone Number
1D5
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:100
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FC
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1:500-1:1,000
Target
Function
Rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR) is a protein that in humans is encoded by the RICTOR gene. RICTOR and mTOR are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth. RICTOR is a subunit of the mammalian target of rapamycin complex 2 (mTORC2) which contains mTOR, GβL, RICTOR (this protein) and mSIN1. The mammalian target of rapamycin (mTOR) is a highly conserved Ser/Thr kinase that regulates cell growth and proliferation. mTOR may exist as mTOR complex 1 (mTORC1) or mTOR complex 2 (mTORC2). RICTOR is a key component of mTORC2, which, unlike mTORC1, is not directly inhibited by rapamycin. mTORC2, and RICTOR, specifically, has been shown to phosphorylate Akt/protein kinase B (PKB) on SER473. This phosphorylation activates Akt/PKB, where deregulation of Akt/PKB has been implicated in cancer and diabetes. RICTOR and mTORC2 have been shown to play an essential role in embryonic growth and development, perhaps due to the control that mTORC2 exerts on actin cytoskeleton organization. Diseases associated with mutation in the RICTOR gene include foramen magnum meningioma and syringomyelia. Akt/PMB activation is also involved in glucose metabolism and activation of Akt by RICTOR has been shown to mediate glucose and lipid metabolism. Therefore, the influence of RICTOR and mTORC2 on Akt signaling has been associated with insulin resistance and type 2 diabetes.
Background References
1. Ramaiah MJ. et. al. mTOR-Rictor-EGFR axis in oncogenesis and diagnosis of glioblastoma multiforme. Mol Biol Rep. 2021 May
2. Xu S. et. al. Rictor Is a Novel Regulator of TRAF6/TRAF3 in Osteoclasts. J Bone Miner Res. 2021 Oct
Subcellular Location
Cytosol, Golgi apparatus.
UNIPROT
Synonyms
AVO3 antibody
AVO3 homolog antibody
DKFZp686B11164 antibody
hAVO3 antibody
KIAA1999 antibody
Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
mAVO3 antibody
MGC39830 antibody
PIA antibody
Pianissimo antibody
ExpandImages
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Western blot analysis of RICTOR on different lysates with Rabbit anti-RICTOR antibody (HA750483) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: SW480 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 192 kDa
Observed band size: 192 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750483) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling RICTOR with Rabbit anti-RICTOR antibody (HA750483) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RICTOR antibody (HA750483) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of Hela cells labeling RICTOR.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750483, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"