KMT3B Recombinant Rabbit Monoclonal Antibody [1B1] - BSA and Azide free
Catalog# HA750507
KMT3B Recombinant Rabbit Monoclonal Antibody [1B1] - BSA and Azide free
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IF-Cell
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Human
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unconjugated
Overview
Product Name
KMT3B Recombinant Rabbit Monoclonal Antibody [1B1] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human KMT3B aa 1-200 / 2,696.
Species Reactivity
Human
Validated Applications
IF-Cell
Target Molecular Weight
Predicted band size: 297 kDa
Positive Control
293.
Conjugation
unconjugated
Clone Number
1B1
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Background References
1. Tauchmann S. et. al. NSD1: A Lysine Methyltransferase between Developmental Disorders and Cancer. Life (Basel). 2021 Aug
2. Yang C. et. al. Role of NSD1 as potential therapeutic target in tumor. Pharmacol Res. 2021 Nov
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
Androgen receptor coactivator 267 kDa protein antibody
Androgen receptor-associated protein of 267 kDa antibody
ARA267 antibody
H3 K36 HMTase antibody
H3-K36-HMTase antibody
H4 K20 HMTase antibody
H4-K20-HMTase antibody
Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific antibody
KMT3B antibody
Lysine N-methyltransferase 3B antibody
ExpandImages
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This data was developed using HA721166, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of 293 cells labeling KMT3B with Rabbit anti-KMT3B antibody (HA721166) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KMT3B antibody (HA721166) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"