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Phospho-eNOS (S1177) Recombinant Rabbit Monoclonal Antibody [PS01-10] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750522

Phospho-eNOS (S1177) Recombinant Rabbit Monoclonal Antibody [PS01-10] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Dog

With BSA and Azide
Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-eNOS (S1177) Recombinant Rabbit Monoclonal Antibody [PS01-10] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser1177 of human eNOS.

Species Reactivity

Human, Dog (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 133 kDa

Positive Control

HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

PS01-10

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme that in humans is encoded by the NOS3 gene located in the 7q35-7q36 region of chromosome 7. This enzyme is one of three isoforms that synthesize nitric oxide (NO), a small gaseous and lipophilic molecule that participates in several biological processes. The other isoforms include neuronal nitric oxide synthase (nNOS), which is constitutively expressed in specific neurons of the brain[8] and inducible nitric oxide synthase (iNOS), whose expression is typically induced in inflammatory diseases. eNOS is primarily responsible for the generation of NO in the vascular endothelium, a monolayer of flat cells lining the interior surface of blood vessels, at the interface between circulating blood in the lumen and the remainder of the vessel wall. NO produced by eNOS in the vascular endothelium plays crucial roles in regulating vascular tone, cellular proliferation, leukocyte adhesion, and platelet aggregation. Therefore, a functional eNOS is essential for a healthy cardiovascular system.

Background References

1. Jin YJ et al. Protein kinase N2 mediates flow-induced endothelial NOS activation and vascular tone regulation. J Clin Invest. 2021 Nov

2. Hebbel RP et al. Multiple inducers of endothelial NOS (eNOS) dysfunction in sickle cell disease. Am J Hematol. 2021 Nov

Subcellular Location

Cell membrane, Membrane, caveola, Cytoplasm, cytoskeleton, Golgi apparatus.

UNIPROT

SwissProt: P29474 Human

Synonyms

cNOS antibody

Constitutive NOS antibody

EC NOS antibody

EC-NOS antibody

ecNOS antibody

Endothelial nitric oxidase synthase antibody

Endothelial nitric oxide synthase antibody

Endothelial nitric oxide synthase 3 antibody

Endothelial NOS antibody

eNOS antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />This data was developed using <a href="/products/HA721188" style="font-weight: bold;text-decoration: underline;">HA721188</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Phospho-eNOS (S1177) on different lysates with Rabbit anti-Phospho-eNOS (S1177) antibody (<a href="/products/HA721188" style="font-weight: bold;text-decoration: underline;">HA721188</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAEC cell lysate (30 µg/Lane)<br />Lane 2: HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate (30 µg/Lane)<br /><br />Predicted band size: 133 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 2 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721188" style="font-weight: bold;text-decoration: underline;">HA721188</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    This data was developed using HA721188, the same antibody clone in a different buffer formulation.
    Western blot analysis of Phospho-eNOS (S1177) on different lysates with Rabbit anti-Phospho-eNOS (S1177) antibody (HA721188) at 1/1,000 dilution.

    Lane 1: HAEC cell lysate (30 µg/Lane)
    Lane 2: HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate (30 µg/Lane)

    Predicted band size: 133 kDa
    Observed band size: 160 kDa

    Exposure time: 2 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721188) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA721188" style="font-weight: bold;text-decoration: underline;">HA721188</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Phospho-eNOS (S1177) antibody (<a href="/products/HA721188" style="font-weight: bold;text-decoration: underline;">HA721188</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721188" style="font-weight: bold;text-decoration: underline;">HA721188</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721188, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Phospho-eNOS (S1177) antibody (HA721188) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721188) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"