TNS4 Recombinant Rabbit Monoclonal Antibody [PD00-95] - BSA and Azide free
Catalog# HA750540
TNS4 Recombinant Rabbit Monoclonal Antibody [PD00-95] - BSA and Azide free
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IHC-P
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Human
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unconjugated
Overview
Product Name
TNS4 Recombinant Rabbit Monoclonal Antibody [PD00-95] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TNS4 aa 700 to the C-terminus (C terminal).
Species Reactivity
Human
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 77 kDa
Positive Control
Human liver cancer tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
PD00-95
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
TNS4, as the others tensins, contains a phosphotyrosine-binding domain (PTB), which plays the role of interacting with the cytoplasmic tail of the β-integrin. Also TNS4 is found to play a central role in HGF-induced tubulogenesis. The role of TNS4 on cell proliferation is found to be minimal and not up to the level of the effect of migration. By enhancing the motility and having the effect on the tubulogenesis, TNS4 is believed to have a role in cancer cell metastasis. The role of cten in cancer is not well defined. In prostate cancer it is down-regulated, where in normal cells it is localized to focal adhesions recruiting the tumour suppressor, deleted in liver cancer (DLC-1), thus suppressing tumorigenesis. Cten in prostate epithelial cells has also been found to regulate staurosporine-induced apoptosis where cten is cleaved by caspase 3 and results in reduction in cell growth rate. Therefore, loss of cten expression may lead to uncontrolled cell growth and result in cell transformation. Accordingly, in prostate cancer, cten may function as a tumour suppressor protein.
Background References
1. Raposo TP et al. Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice. Int J Exp Pathol. 2020 Jun
2. Raposo TP et al. Investigating TNS4 in the Colorectal Tumor Microenvironment Using 3D Spheroid Models of Invasion. Adv Biosyst. 2020 Jun
Subcellular Location
Cell junction, focal adhesion, Cytoplasm, cytoskeleton.
UNIPROT
Synonyms
C terminal tensin like antibody
C terminal tensin like protein antibody
C-terminal tensin-like protein antibody
CTEN antibody
FLJ14950 antibody
PP14434 antibody
TENS4_HUMAN antibody
Tensin 4 antibody
Tensin-4 antibody
Tns4 antibody
Images
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This data was developed using HA721208, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-TNS4 antibody (HA721208) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721208) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721208, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TNS4 antibody (HA721208) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721208) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"