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CD15 Recombinant Rabbit Monoclonal Antibody [PD00-42] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750565

CD15 Recombinant Rabbit Monoclonal Antibody [PD00-42] - BSA and Azide free

Application

  • IHC-P

  • FC

  • IF-Cell

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD15 Recombinant Rabbit Monoclonal Antibody [PD00-42] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Purified CD15 .

Species Reactivity

Human

Validated Applications

IHC-P, FC, IF-Cell

Target Molecular Weight

Predicted band size: 59 kDa

Positive Control

Human tonsil, Human breast carcinoma tissue, human peripheral blood granulocytes, K-562.

Conjugation

unconjugated

Clone Number

PD00-42

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

  • IF-Cell

  • 1:20

Target

Function

CD15 is a complex cluster of cell surface glycoproteins and glycolipids having a common terminal pentasaccaharide known as the Lewisx (Lex) antigen. CD15 is a haemopoietic differentiation antigen expressed on most terminally differentiated myeloid cells including granulocytes, eosinophils, mast cells, monocytes/macrophages, and Langerhans' cells. CD15 is not substantially expressed on haemopoietic progenitor cells. The positivity for CD15 is characteristic of Hodgkin’s cells in classical Hodgkin’s disease (HD). Rare cases of acute lymphoblastic leukaemia, in which myeloid antigens are often CD15 positive. Myeloid leukaemia cells express CD15 in a heterogeneous manner. CMLs are regularly CD15 positive. CD15 is expressed in a varying proportion of epithelial tumours such as adenocarcinomas (particularly from breast, lung and colon), renal cell carcinoma, apocrine carcinoma of the skin, papillary and follicular carcinoma of the thyroid, and serous carcinoma of the ovary. It is possible that sialyl-CD15 confer on the tumour cells the capacity to metastasize. Malignant mesothelioma is practically always CD15 negative (positivity has been reported in up to 6%, particularly the desmoplastic variant). In gliomas, CD15 positivity inversely correlates with the grade of malignancy. Among germ cell tumours, CD15 is detected only in mature teratoma. In haematopathology CD15 is important for the diagnosis of classical HD and characterization of acute leukaemia. CD15 may be used for histopathological grading of gliomas and differentiating between malignant gliomas and non-neoplastic glial cells (the latter usually strongly stained). Kidney and tonsil are recommended as positive and negative tissue controls for CD15.

Background References

1. Seidmann L et al. CD15 immunostaining improves placental diagnosis of fetal hypoxia. Placenta. 2021 Feb

2. Tian X et al. Circulating CD15+ LOX-1+ PMN-MDSCs are a potential biomarker for the early diagnosis of non-small-cell lung cancer. Int J Clin Pract. 2021 Aug

Subcellular Location

Golgi apparatus, Golgi stack membrane

Synonyms

3 fucosyl N acetyl lactosamine antibody

3-FAL antibody

3-fucosyl-N-acetyl-lactosamine antibody

Leu M1 antibody

Lewis X antibody

LeX antibody

SSEA-1 antibody

stage-specific embryonic antigen 1 antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-CD15 antibody (<a href="/products/HA750565" style="font-weight: bold;text-decoration: underline;">HA750565</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750565" style="font-weight: bold;text-decoration: underline;">HA750565</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-CD15 antibody (HA750565) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of human peripheral blood granulocytes labelling CD15 (<a href="/products/HA750565" style="font-weight: bold;text-decoration: underline;">HA750565</a>).

    Flow cytometric analysis of human peripheral blood granulocytes labelling CD15 (HA750565).

  • Immunocytochemistry analysis of K-562 cells labeling CD15 with Rabbit anti-CD15 antibody (<a href="/products/HA750565" style="font-weight: bold;text-decoration: underline;">HA750565</a>) at 1/20 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD15 antibody (<a href="/products/HA750565" style="font-weight: bold;text-decoration: underline;">HA750565</a>) at 1/20 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of K-562 cells labeling CD15 with Rabbit anti-CD15 antibody (HA750565) at 1/20 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD15 antibody (HA750565) at 1/20 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"