VILIP1 Recombinant Rabbit Monoclonal Antibody [PSH0-08] - BSA and Azide free
Catalog# HA750577
VILIP1 Recombinant Rabbit Monoclonal Antibody [PSH0-08] - BSA and Azide free
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
HA721261
含抗保成分
-
unconjugated
Overview
Product Name
VILIP1 Recombinant Rabbit Monoclonal Antibody [PSH0-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human VILIP1 aa 1-191 / 191.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 22 kDa
Positive Control
Human brain tissue lysate, HepG2 cell lysate, HCT116 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, rat cerebellum tissue, human brain tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
PSH0-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Visinin-like protein 1 is a protein that in humans is encoded by the VSNL1 gene. This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
Background References
1. Dai QQ et al. VSNL1 Promotes Gastric Cancer Cell Proliferation and Migration by Regulating P2X3/P2Y2 Receptors and Is a Clinical Indicator of Poor Prognosis in Gastric Cancer Patients. Gastroenterol Res Pract. 2020 Dec
2. He C et al. VSNL1 Promotes Cell Proliferation, Migration, and Invasion in Colorectal Cancer by Binding with COL10A1. Ann Clin Lab Sci. 2022 Jan
Subcellular Location
Cytosol, membrane.
Synonyms
21 kDa CABP antibody
Hippocalcin like protein 3 antibody
Hippocalcin-like protein 3 antibody
HLP 3 antibody
HLP3 antibody
HPCAL 3 antibody
HPCAL3 antibody
HUVISL1 antibody
Neural visinin-like protein 1 antibody
Neural visinin-like type 1 protein antibody
ExpandImages
-
Western blot analysis of VILIP1 on different lysates with Rabbit anti-VILIP1 antibody (HA750577) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: HCT116 cell lysate (10 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of VILIP1 on different lysates with Rabbit anti-VILIP1 antibody (HA750577) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VILIP1 antibody (HA750577) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-VILIP1 antibody (HA750577) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VILIP1 antibody (HA750577) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"