alpha Tubulin Recombinant Rabbit Monoclonal Antibody [PSH0-14] - BSA and Azide free
Catalog# HA750584
alpha Tubulin Recombinant Rabbit Monoclonal Antibody [PSH0-14] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA721272
含抗保成分
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unconjugated
Overview
Product Name
alpha Tubulin Recombinant Rabbit Monoclonal Antibody [PSH0-14] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TUBA3C aa 150-350 / 450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 50 kDa
Positive Control
293T cell lysate, A431 cell lysate, HepG2 cell lysate, 293 cell lysate, Hela cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, human testis tissue.
Conjugation
unconjugated
Clone Number
PSH0-14
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:2,000
Target
Function
Tubulin alpha-3C/D chain is a protein that in humans is encoded by the TUBA3C gene. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene is an alpha tubulin gene that encodes a protein 99% to the mouse testis-specific Tuba3 and Tuba7 gene products. This gene is located in the 13q11 region, which is associated with the genetic diseases Clouston hidrotic ectodermal dysplasia and Kabuki syndrome. Alternative splicing has been observed for this gene and two variants have been identified.
Background References
1. Xu QQ et al. The Expression and Potential Role of Tubulin Alpha 1b in Wilms\' Tumor. Biomed Res Int. 2020 Aug
2. Hu X et al. Tubulin Alpha 1b Is Associated with the Immune Cell Infiltration and the Response of HCC Patients to Immunotherapy. Diagnostics (Basel). 2022 Mar
Subcellular Location
Cytoplasm, cytoskeleton.
Synonyms
Alpha-tubulin 1 antibody
ALS22 antibody
B ALPHA 1 antibody
bA408E5.3 antibody
H2 ALPHA antibody
Hum a tub1 antibody
Hum a tub2 antibody
LIS3 antibody
MGC171407 antibody
MGC55332 antibody
ExpandImages
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Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750584) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750584) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750584) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750584) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750584) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750584) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"