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PBX1 Recombinant Rabbit Monoclonal Antibody [PSH0-24] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750598

PBX1 Recombinant Rabbit Monoclonal Antibody [PSH0-24] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PBX1 Recombinant Rabbit Monoclonal Antibody [PSH0-24] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PBX1 aa 1-250 / 430.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 47 kDa

Positive Control

Hela cell lysate, HEK-293 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, A431 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat pancreas tissue lysate, human pancreas tissue, mouse pancreas tissue.

Conjugation

unconjugated

Clone Number

PSH0-24

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Background References

1. Veiga RN et al. PBX1: a key character of the hallmarks of cancer. J Mol Med (Berl). 2021 Dec

2. Zhou Y et al. PBX1 expression in uterine natural killer cells drives fetal growth. Sci Transl Med. 2020 Apr

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P40424 Human

SwissProt: P41778 Mouse

Entrez Gene: 304947 Rat

Synonyms

DKFZp686B09108 antibody

Homeo box protein PBX1 antibody

Homeo box protein PRL antibody

Homeobox protein PBX 1 antibody

Homeobox protein PBX1 antibody

Homeobox protein PRL antibody

MGC126627 antibody

PBX 1 antibody

Pbx1 antibody

PBX1_HUMAN antibody

Expand

Images

  • This data was developed using <a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (<a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: MCF-7 cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: A431 cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 8: Rat pancreas tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 47 kDa<br />Observed band size: 40/47 kDa<br /><br />Exposure time: 3 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA721303, the same antibody clone in a different buffer formulation.
    Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (HA721303) at 1/1,000 dilution.

    Lane 1: Hela cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: MCF-7 cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: A431 cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: Mouse testis tissue lysate (40 µg/Lane)
    Lane 8: Rat pancreas tissue lysate (40 µg/Lane)

    Predicted band size: 47 kDa
    Observed band size: 40/47 kDa

    Exposure time: 3 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721303) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (<a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721303, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (HA721303) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721303) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-PBX1 antibody (<a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721303" style="font-weight: bold;text-decoration: underline;">HA721303</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721303, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-PBX1 antibody (HA721303) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721303) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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