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SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-25] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750599

SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-25] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-25] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human SEC62 aa 1-50 / 399.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 46 kDa

Positive Control

Hela cell lysate, K562 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, 293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue lysate, mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH0-25

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Translocation protein SEC62 is a protein that in humans is encoded by the SEC62 gene. The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.

Background References

1. Liu X et al. Sec62 promotes stemness and chemoresistance of human colorectal cancer through activating Wnt/β-catenin pathway. J Exp Clin Cancer Res. 2021 Apr

2. Su S et al. Sec62 promotes gastric cancer metastasis through mediating UPR-induced autophagy activation. Cell Mol Life Sci. 2022 Feb

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q99442 Human

SwissProt: Q8BU14 Mouse

Entrez Gene: 294912 Rat

Synonyms

Dtrp1 antibody

FLJ32803 antibody

hTP-1 antibody

HTP1 antibody

Membrane protein SEC62, S.cerevisiae, homolog of antibody

OTTHUMP00000213390 antibody

sec62 antibody

SEC62 homolog (S. cerevisiae) antibody

SEC62_HUMAN antibody

TLOC1 antibody

Expand

Images

  • Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (<a href="/products/HA750599" style="font-weight: bold;text-decoration: underline;">HA750599</a>) at 1/5,000 dilution.<br /><br />Lane 1: Hela cell lysate (20 µg/Lane)<br />Lane 2: Hela cell lysate (20 µg/Lane)<br />Lane 3: K562 cell lysate (20 µg/Lane)<br />Lane 4: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 5: HepG2 cell lysate (20 µg/Lane)<br />Lane 6: 293T cell lysate (20 µg/Lane)<br />Lane 7: Jurkat cell lysate (20 µg/Lane)<br /><br />Predicted band size: 46 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 30 seconds;<br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750599" style="font-weight: bold;text-decoration: underline;">HA750599</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA750599) at 1/5,000 dilution.

    Lane 1: Hela cell lysate (20 µg/Lane)
    Lane 2: Hela cell lysate (20 µg/Lane)
    Lane 3: K562 cell lysate (20 µg/Lane)
    Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 5: HepG2 cell lysate (20 µg/Lane)
    Lane 6: 293T cell lysate (20 µg/Lane)
    Lane 7: Jurkat cell lysate (20 µg/Lane)

    Predicted band size: 46 kDa
    Observed band size: 56 kDa

    Exposure time: 30 seconds;
    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750599) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (<a href="/products/HA750599" style="font-weight: bold;text-decoration: underline;">HA750599</a>) at 1/5,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 2: PC-12 cell lysate (20 µg/Lane)<br />Lane 3: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 4: Rat liver tissue lysate (40 µg/Lane)<br />Lane 5: Human liver tissue lysate (40 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 46 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750599" style="font-weight: bold;text-decoration: underline;">HA750599</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA750599) at 1/5,000 dilution.

    Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 2: PC-12 cell lysate (20 µg/Lane)
    Lane 3: Mouse liver tissue lysate (40 µg/Lane)
    Lane 4: Rat liver tissue lysate (40 µg/Lane)
    Lane 5: Human liver tissue lysate (40 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 46 kDa
    Observed band size: 56 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750599) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"