ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] - BSA and Azide free
Catalog# HA750600
ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] - BSA and Azide free
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ASC1 aa 1-581 / 581.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 66 kDa
Positive Control
A549 cell lysate, Hela cell lysate, U87-MG cell lysate, 293T cell lysate, HL-60 cell lysate, HepG2 cell lysate, RH-35 cell lysate, HeLa cell lysate, Rat testis tissue lysate, rat small intestine tissue.
Conjugation
unconjugated
Clone Number
PSH0-26
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1).
Background References
1. Dembour A et al. ASC1 complex related conditions: Two novel paediatric patients with TRIP4 pathogenic variants and review of literature. Eur J Med Genet. 2022 Apr
Subcellular Location
Nucleus, Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Synonyms
Activating signal cointegrator 1 antibody
ASC 1 antibody
ASC-1 antibody
HsT17391 antibody
Thyroid hormone receptor interactor 4 antibody
Thyroid receptor interacting protein 4 antibody
Thyroid receptor-interacting protein 4 antibody
TR-interacting protein 4 antibody
TRIP 4 antibody
TRIP-4 antibody
ExpandImages
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This data was developed using HA721306, the same antibody clone in a different buffer formulation.
Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA721306) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: U87-MG cell lysate
Lane 4: 293T cell lysate
Lane 5: HL-60 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: RH-35 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 3 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721306) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721306, the same antibody clone in a different buffer formulation.
Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA721306) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721306, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (HA721306) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721306) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ASC1 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Rat
Conjugate: unconjugated
