LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH0-42] - BSA and Azide free
Catalog# HA750620
LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH0-42] - BSA and Azide free
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WB
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IF-Cell
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Mouse
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HA721344
含抗保成分
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unconjugated
Overview
Product Name
LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH0-42] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human LAG-3 aa 1-500 / 525.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 57.4 kDa
Positive Control
NIH/3T3 transfected with LAG3 cell lysate, NIH/3T3 transfected with LAG3.
Conjugation
unconjugated
Clone Number
PSH0-42
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
Target
Function
Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene. LAG3, which was discovered in 1990 and was designated CD223 (cluster of differentiation 223) after the Seventh Human Leucocyte Differentiation Antigen Workshop in 2000, is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders. In soluble form it is also being developed as a cancer drug in its own right. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function. Fibrinogen-like protein1 FGL1, a liver-secreted protein, is another (major) LAG3 functional ligand independent of MHC-II. LAG3 also helps maintain CD8+ T cells in a tolerogenic state and, working with PD-1, helps maintain CD8 exhaustion during chronic viral infection. LAG3 is known to be involved in the maturation and activation of dendritic cells.
Background References
1. Chocarro L et al. Understanding LAG-3 Signaling. Int J Mol Sci. 2021 May
2. Maruhashi T et al. LAG-3: from molecular functions to clinical applications. J Immunother Cancer. 2020 Sep
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD223 antibody
CD223 antigen antibody
FDC protein antibody
LAG-3 antibody
Lag3 antibody
LAG3_HUMAN antibody
Lymphocyte activating 3 antibody
Lymphocyte activation gene 3 antibody
Lymphocyte activation gene 3 protein antibody
Protein FDC antibody
Images
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☑ Cell treatment (CT)
Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (HA750620) at 1/2,000 dilution.
Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate
Lane 2: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57.4 kDa
Observed band size: 55/70 kDa (Glycosylation)
Exposure time: 1 minute 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750620) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (HA750620) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (HA750620) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"