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LXR beta Recombinant Rabbit Monoclonal Antibody [PSH0-56] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750633

LXR beta Recombinant Rabbit Monoclonal Antibody [PSH0-56] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

LXR beta Recombinant Rabbit Monoclonal Antibody [PSH0-56] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human LXR beta aa 50-100/460.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 51 kDa

Positive Control

SW480 cell lysate, HepG2 cell lysate, PC-3M cell lysate, A549 cell lysate, HeLa cell lysate, HT-29 cell lysate, HCT116 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, C2C12, HCT116.

Conjugation

unconjugated

Clone Number

PSH0-56

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Liver X receptor beta (LXR-β) is a member of the nuclear receptor family of transcription factors. LXR-β is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides. Two genes, alpha (LXRA) and beta, are known to encode LXR proteins. Crystal structure of human liver X receptor β(LXRβ) forming heterodimer with its partner retinoid X receptor α(RXRα) on its cognate element, an AGGTCA direct repeat spaced by 4 nt shows an extended X-shaped arrangement, with DNA- and ligand-binding domains crossed. The LXRβ core binds DNA via canonical contacts and auxiliary DNA contacts that enhance affinity for the response element. Liver X receptor beta has been shown to interact with NCOA6[8] and Retinoid X receptor alpha.

Background References

1. Zhang W et al. The LXRB-SREBP1 network regulates lipogenic homeostasis by controlling the synthesis of polyunsaturated fatty acids in goat mammary epithelial cells. J Anim Sci Biotechnol. 2022 Nov

2. Wolski H et al. Expression of ABCA1 Transporter and LXRA/LXRB Receptors in Placenta of Women with Late Onset Preeclampsia. J Clin Med. 2022 Aug

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P55055 Human

SwissProt: Q60644 Mouse

SwissProt: Q62755 Rat

Synonyms

Liver X nuclear receptor beta antibody

Liver X receptor beta antibody

LX receptor beta antibody

LXR b antibody

LXR beta antibody

LXRB antibody

NER antibody

NER I antibody

NER1 antibody

NR1H 2 antibody

Expand

Images

  • Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: PC-3M cell lysate (20 µg/Lane)<br />Lane 4: A549 cell lysate (20 µg/Lane)<br />Lane 5: HeLa cell lysate (20 µg/Lane)<br />Lane 6: HT-29 cell lysate (20 µg/Lane)<br />Lane 7: HCT116 cell lysate (20 µg/Lane)<br />Lane 8: HEK-293 cell lysate (20 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 10: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 11: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (HA750633) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: PC-3M cell lysate (20 µg/Lane)
    Lane 4: A549 cell lysate (20 µg/Lane)
    Lane 5: HeLa cell lysate (20 µg/Lane)
    Lane 6: HT-29 cell lysate (20 µg/Lane)
    Lane 7: HCT116 cell lysate (20 µg/Lane)
    Lane 8: HEK-293 cell lysate (20 µg/Lane)
    Lane 9: Mouse brain tissue lysate (40 µg/Lane)
    Lane 10: Mouse liver tissue lysate (40 µg/Lane)
    Lane 11: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750633) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of C2C12 cells labeling LXR beta with Rabbit anti-LXR beta antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of C2C12 cells labeling LXR beta with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of HCT116 cells labeling LXR beta with Rabbit anti-LXR beta antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HCT116 cells labeling LXR beta with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293-si NT cell lysate<br />Lane 2: HEK-293-si LXR beta cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750633" style="font-weight: bold;text-decoration: underline;">HA750633</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (HA750633) at 1/1,000 dilution.

    Lane 1: HEK-293-si NT cell lysate
    Lane 2: HEK-293-si LXR beta cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750633) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

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