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Cystathionase Recombinant Rabbit Monoclonal Antibody [JE35-91] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750649

Cystathionase Recombinant Rabbit Monoclonal Antibody [JE35-91] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Cystathionase Recombinant Rabbit Monoclonal Antibody [JE35-91] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Cystathionase aa 1-405 / 405.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HepG2 cell lysate, 293T cell lysate, K-562 cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate,.

Conjugation

unconjugated

Clone Number

JE35-91

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:10,000

  • IP

  • 1-2μg/sample

Target

Function

The enzyme cystathionine γ-lyase (EC 4.4.1.1, CTH or CSE; also cystathionase; systematic name L-cystathionine cysteine-lyase (deaminating; 2-oxobutanoate-forming)) breaks down cystathionine into cysteine, 2-oxobutanoate (α-ketobutyrate), and ammonia. In some bacteria and mammals, including humans, this enzyme takes part in generating hydrogen sulfide. Hydrogen sulfide is one of a few gases that was recently discovered to have a role in cell signaling in the body. H2S decreases transcription of cystathionase at concentrations between 10 and 80μM. However, transcription is increased by concentrations near 120μM, and inhibited completely at concentrations in excess of 160μM.

Background References

1. Xu W et al. Hepatocellular cystathionine γ lyase/hydrogen sulfide attenuates nonalcoholic fatty liver disease by activating farnesoid X receptor. Hepatology. 2022 Dec

2. Wu H et al. Modification of mesenchymal stem cells by HMGB1 promotes the activity of Cav3.2 T-type calcium channel via PKA/β-catenin/γ-cystathionase pathway. Stem Cell Res Ther. 2022 Jan

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P32929 Human

SwissProt: Q8VCN5 Mouse

SwissProt: P18757 Rat

Synonyms

CGL_HUMAN antibody

CTH antibody

cystathionase (cystathionine gamma-lyase) antibody

Cystathionine gamma lyase antibody

Cystathionine gamma-lyase antibody

Cysteine desulfhydrase antibody

Gamma cystathionase antibody

Gamma-cystathionase antibody

Homoserine deaminase antibody

Homoserine dehydratase antibody

Expand

Images

  • Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (<a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: Human liver tissue lysate (40 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 6: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA750649) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: Human liver tissue lysate (40 µg/Lane)
    Lane 5: Mouse liver tissue lysate (40 µg/Lane)
    Lane 6: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 4 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750649) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with <a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A431 cell lysate (input)<br />Lane 2: <a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a> IP in A431 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a> in A431 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 24 seconds

    Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with HA750649 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750649 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A431 cell lysate (input)
    Lane 2: HA750649 IP in A431 cell lysate
    Lane 3: Rabbit IgG instead of HA750649 in A431 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 24 seconds

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (<a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a>) at 1/10,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Cystathionase KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750649" style="font-weight: bold;text-decoration: underline;">HA750649</a>) at 1/10,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA750649) at 1/10,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Cystathionase KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750649) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"