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Ms4a10 Recombinant Rabbit Monoclonal Antibody [PSH0-71] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750650

Ms4a10 Recombinant Rabbit Monoclonal Antibody [PSH0-71] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Ms4a10 Recombinant Rabbit Monoclonal Antibody [PSH0-71] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within mouse Ms4a10 aa 218-267 / 267.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 29 kDa

Positive Control

Mouse small intestine tissue, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

PSH0-71

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:100-1:500

  • IF-Tissue

  • 1:200

Target

Function

Most MS4A genes, including MS4A10, encode proteins with at least 4 potential transmembrane domains and N- and C-terminal cytoplasmic domains encoded by distinct exons. May be involved in signal transduction as a component of a multimeric receptor complex.

Background References

暂无

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q99N03 Mouse

SwissProt: D4A504 Rat

Synonyms

Membrane-spanning 4-domains subfamily A member 10

Ms4a10

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Ms4a10 on different lysates with Rabbit anti-Ms4a10 antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa whole cell lysate<br />Lane 2: HeLa overexpress mouse Ms4a10 whole cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29/25 kDa<br /><br />Exposure time: 4 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Ms4a10 on different lysates with Rabbit anti-Ms4a10 antibody (HA750650) at 1/1,000 dilution.

    Lane 1: HeLa whole cell lysate
    Lane 2: HeLa overexpress mouse Ms4a10 whole cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 29/25 kDa

    Exposure time: 4 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Ms4a10 antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Ms4a10 antibody (HA750650) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750650) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Ms4a10 antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Ms4a10 antibody (HA750650) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750650) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.<br /><br />Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (HA750650) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

    Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of paraffin-embedded rat small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA750650" style="font-weight: bold;text-decoration: underline;">HA750650</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.<br /><br />Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded rat small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (HA750650) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

    Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"