Syndecan 4 Recombinant Rabbit Monoclonal Antibody [PSH0-72] - BSA and Azide free
Catalog# HA750651
Syndecan 4 Recombinant Rabbit Monoclonal Antibody [PSH0-72] - BSA and Azide free
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WB
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Human
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HA721473
含抗保成分
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unconjugated
Overview
Product Name
Syndecan 4 Recombinant Rabbit Monoclonal Antibody [PSH0-72] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Syndecan 4 Extracellular.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 22 kDa
Positive Control
HepG2 cell lysate, Jurkat cell lysate, HeLa cell lysate, U-87 MG cell lysate, A549 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate.
Conjugation
unconjugated
Clone Number
PSH0-72
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Syndecan-4 is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. Syndecan-4 interacts with extracellular matrix, anticoagulants, and growth-factors. It also regulates the actin cytoskeleton, cell adhesion, and cell migration. Syndecan-4 activates protein kinase C (PKC), an enzyme involved in signal transduction. The variable domain of syndecan-4 could be a site of self-association. The degree of oligomerization correlates with the activity of kinases, so the degree of clustering of syndecan-4 correlates to PKC activity. Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold. Syndecan-4 is also a regulator of fibroblast growth factor-2 (FGF-2) signaling. Syndecan-4 binds to FGF and mediates interaction with the FGF receptor. Because the tight correlation between syndecan-4 and growth factors, the efficiency of angiogenic therapies have been thought to relate to syndecan-4. Growth factor signaling may be disrupted by changes in syndecan-4 expression. The cellular uptake, trafficking, and nuclear localization of FGF-2 could be increased by co-delivery of syndecan-4 proteoliposomes. These alterations should be considered in FGF-2-based therapies. Syndecan-4 is also associated with the healing process. Lack of Sdc4 gene causes delayed wound healing in mice. This delay may be due to compromised fibroblast motility.
Background References
1. Onyeisi JOS et al. Syndecan-4 as a Pathogenesis Factor and Therapeutic Target in Cancer. Biomolecules. 2021 Mar
2. Keller-Pinter A et al. Syndecan-4 in Tumor Cell Motility. Cancers (Basel). 2021 Jul
Subcellular Location
Membrane, Secreted.
UNIPROT
Synonyms
Amphiglycan antibody
MGC22217 antibody
OTTHUMP00000031788 antibody
ryudocan amphiglycan antibody
Ryudocan antibody
Ryudocan core protein antibody
SDC 4 antibody
Sdc4 antibody
SDC4_HUMAN antibody
SYND 4 antibody
ExpandImages
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Western blot analysis of Syndecan 4 on different lysates with Rabbit anti-Syndecan 4 antibody (HA750651) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: A549 cell lysate
Lane 6: MCF7 cell lysate
Lane 7: MDA-MB-231 cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750651) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"