Syntaxin 4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH0-80] (HA750660)
Catalog# HA750660
Rabbit Monoclonal to Syntaxin 4
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (HA750660) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: HCT 116 cell lysate (20 µg/Lane)
Lane 8: U-87 MG cell lysate (20 µg/Lane)
Lane 9: HUVEC cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: RAW264.7 cell lysate (20 µg/Lane)
Lane 12: PC-12 cell lysate (20 µg/Lane)
Lane 13: Mouse kidney tissue lysate (40 µg/Lane)
Lane 14: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: HCT 116 cell lysate (20 µg/Lane)
Lane 8: U-87 MG cell lysate (20 µg/Lane)
Lane 9: HUVEC cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: RAW264.7 cell lysate (20 µg/Lane)
Lane 12: PC-12 cell lysate (20 µg/Lane)
Lane 13: Mouse kidney tissue lysate (40 µg/Lane)
Lane 14: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.