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TDP1 Recombinant Rabbit Monoclonal Antibody [PSH0-93] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750673

TDP1 Recombinant Rabbit Monoclonal Antibody [PSH0-93] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TDP1 Recombinant Rabbit Monoclonal Antibody [PSH0-93] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TDP1 aa 100-608 / 608.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 68 kDa

Positive Control

MCF7 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A375 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue.

Conjugation

unconjugated

Clone Number

PSH0-93

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Tyrosyl-DNA phosphodiesterase 1 is an enzyme that in humans is encoded by the TDP1 gene. The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of Type I topoisomerase and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

Background References

1. Zhang H et al. TDP1-independent pathways in the process and repair of TOP1-induced DNA damage. Nat Commun. 2022 Jul

2. Jakobsen AK et al. TDP1 and TOP1 as targets in anticancer treatment of NSCLC: Activity and protein level in normal and tumor tissue from 150 NSCLC patients correlated to clinical data. Lung Cancer. 2022 Feb

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9NUW8 Human

SwissProt: Q8BJ37 Mouse

SwissProt: Q4G056 Rat

Synonyms

AI838772 antibody

AW493413 antibody

FLJ11090 antibody

MGC104252 antibody

MGC112732 antibody

RP24-311F12.2 antibody

SCAN1 antibody

TDP1 antibody

TYDP antibody

TYDP1_HUMAN antibody

Expand

Images

  • This data was developed using <a href="/products/HA721531" style="font-weight: bold;text-decoration: underline;">HA721531</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of TDP1 on different lysates with Rabbit anti-TDP1 antibody (<a href="/products/HA721531" style="font-weight: bold;text-decoration: underline;">HA721531</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: A375 cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 68 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721531" style="font-weight: bold;text-decoration: underline;">HA721531</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA721531, the same antibody clone in a different buffer formulation.
    Western blot analysis of TDP1 on different lysates with Rabbit anti-TDP1 antibody (HA721531) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: A375 cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721531) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA721531" style="font-weight: bold;text-decoration: underline;">HA721531</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TDP1 antibody (<a href="/products/HA721531" style="font-weight: bold;text-decoration: underline;">HA721531</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721531" style="font-weight: bold;text-decoration: underline;">HA721531</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721531, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TDP1 antibody (HA721531) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721531) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"