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SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750691

SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human SMARCA2 aa 231-280 / 1,590.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 181 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, MCF7 cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human prostate carcinoma tissue, MCF7.

Conjugation

unconjugated

Clone Number

PSH01-15

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • FC

  • 1:1,000

Target

Function

Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Background References

1. Cantley J et al. Selective PROTAC-mediated degradation of SMARCA2 is efficacious in SMARCA4 mutant cancers. Nat Commun. 2022 Nov

2. Kofink C et al. A selective and orally bioavailable VHL-recruiting PROTAC achieves SMARCA2 degradation in vivo. Nat Commun. 2022 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P51531 Human

Synonyms

ATP dependent helicase SMARCA2 antibody

ATP-dependent helicase SMARCA2 antibody

BAF190 antibody

BAF190B antibody

BRG1-associated factor 190B antibody

BRM antibody

FLJ36757 antibody

Global transcription activator homologous sequence antibody

hBRM antibody

hSNF2a antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: NCCIT cell lysate (low expression)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 181 kDa<br />Observed band size: 200 kDa<br /><br />Exposure time: 3 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (HA750691) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: NCCIT cell lysate (low expression)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 181 kDa
    Observed band size: 200 kDa

    Exposure time: 3 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750691) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMARCA2 antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMARCA2 antibody (HA750691) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SMARCA2 antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SMARCA2 antibody (HA750691) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of MCF7 cells labeling SMARCA2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA750691" style="font-weight: bold;text-decoration: underline;">HA750691</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of MCF7 cells labeling SMARCA2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA750691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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