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MSI2 Recombinant Rabbit Monoclonal Antibody [PSH01-17] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750693

MSI2 Recombinant Rabbit Monoclonal Antibody [PSH01-17] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Cell

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

MSI2 Recombinant Rabbit Monoclonal Antibody [PSH01-17] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MSI2 aa 1-185 / 328.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, IP

Molecular Weight

Predicted band size: 35 kDa

Positive Control

PC-12 cell lysate, K-562 cell lysate, human placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat testis tissue lysate, mouse brain tissue, rat brain tissue, HeLa.

Conjugation

unconjugated

Clone Number

PSH01-17

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:100

  • IP

  • 1-2μg/sample

Target

Function

This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is translational regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells. The first musashi (abbreviation MSI) gene was first discovered in Drosophila and then later identified in other eukaryotic species. MSI2 is involved in organismal development. As with the rest of Musashi family RNA-binding proteins, MSI2 is linked to tissue stem cells and has an influence in asymmetric cell division, germ and somatic stem cell function and cell fate determination in a variety of tissues. As an RNA-binding protein, MSI2 is acts as a translational inhibitor. Through this molecular mechanism, MSI2 contributes in more than one vital aspect, as in the development of the nervous system, regulation of the Hematopoietic stem cell (HSC) compartment, or the self-renewal and pluripotency of embryonic stem cells. MSI2 takes part in a high number of pathways related to the self-renewal of some stem cells. However, it is not only focused in one specific type. Depending on the tissue where it is located, it develops different functions.

Background References

1. Zhu Y et al. LncRNA LINC00942 promotes chemoresistance in gastric cancer by suppressing MSI2 degradation to enhance c-Myc mRNA stability. Clin Transl Med. 2022 Jan

2. Qu C et al. Myofibroblast-Specific Msi2 Knockout Inhibits HCC Progression in a Mouse Model. Hepatology. 2021 Jul

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q96DH6 Human

SwissProt: Q920Q6 Mouse

Entrez Gene: 360596 Rat

Synonyms

FLJ36569 antibody

MGC3245 antibody

Msi2 antibody

MSI2/HOXA9 fusion gene, included antibody

MSI2H antibody

MSI2H_HUMAN antibody

Musashi 2 antibody

Musashi homolog 2 antibody

Musashi RNA binding protein 2 antibody

Musashi, Drosophila, homolog of, 2 antibody

Expand

Images

  • Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: Human placenta tissue lysate (40 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 5: Rat brain tissue lysate (40 µg/Lane)<br />Lane 6: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35/37 kDa<br />Exposure time: 3 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (HA750693) at 1/1,000 dilution.

    Lane 1: PC-12 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: Human placenta tissue lysate (40 µg/Lane)
    Lane 4: Mouse brain tissue lysate (40 µg/Lane)
    Lane 5: Rat brain tissue lysate (40 µg/Lane)
    Lane 6: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 35 kDa
    Observed band size: 35/37 kDa
    Exposure time: 3 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750693) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (HA750693) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750693) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MSI2 antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MSI2 antibody (HA750693) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750693) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of HeLa cells labeling MSI2 with Rabbit anti-MSI2 antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody (<a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling MSI2 with Rabbit anti-MSI2 antibody (HA750693) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody (HA750693) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • MSI2 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA750693" style="font-weight: bold;text-decoration: underline;">HA750693</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 12 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    MSI2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750693 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750693 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA750693 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA750693 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 12 seconds; ECL: K1801

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