HADHA Recombinant Rabbit Monoclonal Antibody [PSH01-29] - BSA and Azide free
Catalog# HA750707
HADHA Recombinant Rabbit Monoclonal Antibody [PSH01-29] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
HADHA Recombinant Rabbit Monoclonal Antibody [PSH01-29] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human HADHA aa 1-763 / 763.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
293T cell lysate, HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, human kidney tissue lysate, rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
PSH01-29
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Trifunctional enzyme subunit alpha, mitochondrial also known as hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit is a protein that in humans is encoded by the HADHA gene. Mutations in HADHA have been associated with trifunctional protein deficiency or long-chain 3-hydroxyacyl-coenzyme A dehydrogenase deficiency. This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The enzyme converts medium- and long-chain 2-enoyl-CoA compounds into the following 3-ketoacyl-CoA when NAD is solely present, and acetyl-CoA when NAD and CoASH are present. The alpha subunit catalyzes this reaction, and is attached to HADHB, which catalyzes the last step of the reaction.
Background References
1. Pan A et al. The mitochondrial β-oxidation enzyme HADHA restrains hepatic glucagon response by promoting β-hydroxybutyrate production. Nat Commun. 2022 Jan
2. Liu Y et al. MiR-612 regulates invadopodia of hepatocellular carcinoma by HADHA-mediated lipid reprogramming. J Hematol Oncol. 2020 Feb
Subcellular Location
Mitochondrion, Mitochondrion inner membrane.
Synonyms
3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit antibody
3 oxoacyl CoA thiolase antibody
78 kDa gastrin binding protein antibody
78 kDa gastrin-binding protein antibody
ECHA antibody
ECHA_HUMAN antibody
GBP antibody
HADH antibody
HADHA antibody
Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit antibody
ExpandImages
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Western blot analysis of HADHA on different lysates with Rabbit anti-HADHA antibody (HA750707) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: COS-1 cell lysate
Lane 7: Mouse heart tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Human kidney tissue lysate
Lane 10: Rat liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 74 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750707) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"