Jarid2 Recombinant Rabbit Monoclonal Antibody [PSH01-32] - BSA and Azide free
Catalog# HA750710
Jarid2 Recombinant Rabbit Monoclonal Antibody [PSH01-32] - BSA and Azide free
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IHC-P
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
Jarid2 Recombinant Rabbit Monoclonal Antibody [PSH01-32] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Jarid2 aa 501-1,050 / 1,246.
Species Reactivity
Human, Mouse
Validated Applications
IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 139 kDa
Positive Control
NCCIT, human cervix tissue, human placenta tissue, human thyroid tissue, mouse esophagus tissue.
Conjugation
unconjugated
Clone Number
PSH01-32
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Protein Jumonji is a protein that in humans is encoded by the JARID2 gene. JARID2 is a member of the alpha-ketoglutarate-dependent hydroxylase superfamily. Jarid2 (jumonji, AT rich interactive domain 2) is a protein coding gene that functions as a putative transcription factor. Distinguished as a nuclear protein necessary for mouse embryogenesis, Jarid2 is a member of the jumonji family that contains a DNA binding domain known as the AT-rich interaction domain (ARID). In vitro studies of Jarid2 reveal that ARID along with other functional domains are involved in DNA binding, nuclear localization, transcriptional repression, and recruitment of Polycomb-repressive complex 2 (PRC2). Intracellular mechanisms underlying these interactions remain largely unknown. In search of developmentally important genes, Jarid2 has previously been identified by gene trap technology as an important factor necessary for organ development. During mouse organogenesis, Jarid2 is involved in the formation of the neural tube and development of the liver, spleen, thymus and cardiovascular system. Continuous Jarid2 expression in the tissues of the heart, highlight its presiding role in the development of both the embryonic and the adult heart. Mutant models of Jarid2 embryos show severe heart malformations, ventricular septal defects, noncompaction of the ventricular wall, and atrial enlargement. Homozygous mutants of Jarid2 are found to die soon after birth. Overexpression of the mouse Jarid2 gene has been reported to repress cardiomyocyte proliferation through it close interaction with retinoblastoma protein (Rb), a master cell cycle regulator. Retinoblastoma-binding protein-2 and the human SMCX protein share regions of homology between mice and humans.
Background References
1. Kasinath V et al. JARID2 and AEBP2 regulate PRC2 in the presence of H2AK119ub1 and other histone modifications. Science. 2021 Jan
2. Verberne EA et al. JARID2 haploinsufficiency is associated with a clinically distinct neurodevelopmental syndrome. Genet Med. 2021 Feb
Subcellular Location
Nucleus.
Synonyms
JARD2 antibody
JARD2_HUMAN antibody
JARID2 antibody
JMJ antibody
Jumonji AT rich interactive domain 2 antibody
Jumonji homolog antibody
Jumonji like protein antibody
Jumonji protein antibody
Jumonji/ARID domain containing protein 2 antibody
Jumonji/ARID domain-containing protein 2 antibody
ExpandImages
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This data was developed using HA721656, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NCCIT cells labeling Jarid2 with Rabbit anti-Jarid2 antibody (HA721656) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jarid2 antibody (HA721656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using HA721656, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jarid2 antibody (HA721656) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721656) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721656, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Jarid2 antibody (HA721656) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721656, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Jarid2 antibody (HA721656) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721656, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-Jarid2 antibody (HA721656) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"