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TMP21 Recombinant Rabbit Monoclonal Antibody [PSH01-40] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750718

TMP21 Recombinant Rabbit Monoclonal Antibody [PSH01-40] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TMP21 Recombinant Rabbit Monoclonal Antibody [PSH01-40] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TMP21 aa 1-200 / 219.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 25 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, Raji cell lysate, Jurkat cell lysate, HepG2 cell lysate, Ramos cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse pancreas tissue lysate, mouse liver tissue lysate, rat pancreas tissue lysate, rat liver tissue lysate, human breast tissue, human endometrium tissue, mouse pancreas tissue, rat pancreas tissue.

Conjugation

unconjugated

Clone Number

PSH01-40

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Transmembrane emp24 domain-containing protein 10 is a protein that in humans is encoded by the TMED10 gene. This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8.

Background References

1. Qiu K et al. TMP21 in Alzheimer\'s Disease: Molecular Mechanisms and a Potential Target. Front Cell Neurosci. 2019 Jul

2. Shin JH et al. TMP21 regulates autophagy by modulating ROS production and mTOR activation. Biochem Biophys Res Commun. 2019 Oct

Subcellular Location

Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Golgi apparatus, cis-Golgi network membrane, Golgi apparatus, trans-Golgi network membrane, Cytoplasmic vesicle, secretory vesicle membrane, Cell membrane, Melanosome.

UNIPROT

SwissProt: P49755 Human

SwissProt: Q9D1D4 Mouse

SwissProt: Q63584 Rat

Synonyms

1110014C03Rik antibody

21 kDa transmembrane trafficking protein antibody

21 kDa transmembrane-trafficking protein antibody

MGC102351 antibody

p23 antibody

p24 family protein delta 1 antibody

p24 family protein delta-1 antibody

p24(DELTA) antibody

p24delta antibody

p24delta1 antibody

Expand

Images

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of TMP21 on different lysates with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: HepG2 cell lysate (20 µg/Lane)<br />Lane 6: Ramos cell lysate (20 µg/Lane)<br />Lane 7: U-87 MG cell lysate (20 µg/Lane)<br />Lane 8: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 9: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br />Lane 11: Mouse pancreas tissue lysate (40 µg/Lane)<br />Lane 12: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 13: Rat pancreas tissue lysate (40 µg/Lane)<br />Lane 14: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 19 kDa<br /><br />Exposure time: 2 minutes 13 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Western blot analysis of TMP21 on different lysates with Rabbit anti-TMP21 antibody (HA721678) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: HepG2 cell lysate (20 µg/Lane)
    Lane 6: Ramos cell lysate (20 µg/Lane)
    Lane 7: U-87 MG cell lysate (20 µg/Lane)
    Lane 8: RAW264.7 cell lysate (20 µg/Lane)
    Lane 9: Neuro-2a cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)
    Lane 11: Mouse pancreas tissue lysate (40 µg/Lane)
    Lane 12: Mouse liver tissue lysate (40 µg/Lane)
    Lane 13: Rat pancreas tissue lysate (40 µg/Lane)
    Lane 14: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 19 kDa

    Exposure time: 2 minutes 13 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721678) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721678) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721678) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721678) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-TMP21 antibody (HA721678) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721678) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Immunofluorescence analysis of paraffin-embedded human breast tissue labeling TMP21 with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling TMP21 with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721678, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • This data was developed using <a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, the same antibody clone in a different buffer formulation.<br />Immunofluorescence analysis of paraffin-embedded rat pancreas tissue labeling TMP21 with Rabbit anti-TMP21 antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721678" style="font-weight: bold;text-decoration: underline;">HA721678</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    This data was developed using HA721678, the same antibody clone in a different buffer formulation.
    Immunofluorescence analysis of paraffin-embedded rat pancreas tissue labeling TMP21 with Rabbit anti-TMP21 antibody (HA721678) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721678, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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