SIAH1 Recombinant Rabbit Monoclonal Antibody [PSH01-80] - BSA and Azide free
Catalog# HA750754
SIAH1 Recombinant Rabbit Monoclonal Antibody [PSH01-80] - BSA and Azide free
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SIAH1 Recombinant Rabbit Monoclonal Antibody [PSH01-80] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SIAH1 aa 1-50 / 282.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
HCT 116 cell lysate, RAW264.7 cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, rat liver tissue lysate, rat brain tissue lysate, Rat testis testis lysate, A549.
Conjugation
unconjugated
Clone Number
PSH01-80
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes.Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2 .Induces apoptosis in cooperation with PEG3 (By similarity). Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus (By similarity). GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins (By similarity). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1
Background References
1. Zhang H, Wang J, Ge Y, Ye M, Jin X. Siah1 in cancer and nervous system diseases (Review). Oncol Rep. 2022 Feb;47(2):35. doi: 10.3892/or.2021.8246. Epub 2021 Dec 27. PMID: 34958110.
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
E3 ubiquitin-protein ligase SIAH1 antibody
FLJ08065 antibody
hSIAH1 antibody
HUMSIAH antibody
Seven in absentia homolog 1 (Drosophila) antibody
Seven in absentia homolog 1 antibody
Siah 1 antibody
Siah 1a antibody
Siah E3 ubiquitin protein ligase 1 antibody
Siah-1 antibody
ExpandImages
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This data was developed using HA721732, the same antibody clone in a different buffer formulation.
Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (HA721732) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate（20 µg/Lane）
Lane 2: RAW264.7 cell lysate（20 µg/Lane）
Lane 3: Mouse liver tissue lysate（40 µg/Lane）
Lane 4: Mouse brain tissue lysate（40 µg/Lane）
Lane 5: Rat liver tissue lysate（40 µg/Lane）
Lane 6: Rat brain tissue lysate（40 µg/Lane）
Lane 7: Rat testis testis lysate（40 µg/Lane）
Predicted band size: 31 kDa
Observed band size: 25 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721732) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721732, the same antibody clone in a different buffer formulation.
Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (HA721732) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate
Lane 3: A549 cell lysate
Lane 4: HCT116 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31/25kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721732) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721732, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (HA721732) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (HA721732) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SIAH1 Recombinant Rabbit Monoclonal Antibody [PSH01-80]
Application: WB,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
SIAH1 Recombinant Rabbit Monoclonal Antibody [PSH0-46]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
