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eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750763

eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human eIF4A2 aa 1-407 / 407.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 46 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, PC-3M cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human brain tissue lysate, mouse testis tissue lysate, rat testis tissue lysate.

Conjugation

unconjugated

Clone Number

PSH01-89

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Background References

1. Liu L. et al. 2022.The splicing factor RBM17 drives leukemic stem cell maintenance by evading nonsense-mediated decay of pro-leukemic factors. Nat Commun. 13(1):3833.

2. Chen Z.H. et al. 2019. Eukaryotic initiation factor 4A2 promotes experimental metastasis and oxaliplatin resistance in colorectal cancer. J Exp Clin Cancer Res.38(1):196.

Subcellular Location

Cytosol.

UNIPROT

SwissProt:Q14240 Human

SwissProt:P10630 Mouse

SwissProt:Q5RKI1 Rat

Synonyms

ATP dependent RNA helicase eIF4A 2 antibody

ATP-dependent RNA helicase eIF4A-2 antibody

BM-010 antibody

DDX2B antibody

eIF 4A II antibody

eIF-4A-II antibody

EIF4A antibody

eIF4A II antibody

eIF4A-II antibody

EIF4A2 antibody

Expand

Images

  • This data was developed using <a href="/products/HA721747" style="font-weight: bold;text-decoration: underline;">HA721747</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (<a href="/products/HA721747" style="font-weight: bold;text-decoration: underline;">HA721747</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: PC-3M cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: MCF7 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: C2C12 cell lysate<br />Lane 8: PC-12 cell lysate<br />Lane 9: COS-1 cell lysate<br />Lane 10: Human brain tissue lysate<br />Lane 11: Mouse testis tissue lysate<br />Lane 12: Rat testis tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 46 kDa<br /><br />Exposure time: 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721747" style="font-weight: bold;text-decoration: underline;">HA721747</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA721747, the same antibody clone in a different buffer formulation.
    Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (HA721747) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: PC-3M cell lysate
    Lane 4: 293T cell lysate
    Lane 5: MCF7 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: C2C12 cell lysate
    Lane 8: PC-12 cell lysate
    Lane 9: COS-1 cell lysate
    Lane 10: Human brain tissue lysate
    Lane 11: Mouse testis tissue lysate
    Lane 12: Rat testis tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

    Exposure time: 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721747) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"