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SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-90] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750764

SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-90] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-90] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SHARP2 aa 151-412 / 412.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 46 kDa

Positive Control

MCF7 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, A431 cell lysate, MCF7, human bladder tissue.

Conjugation

unconjugated

Clone Number

PSH01-90

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation.

Background References

1. Wang L et al. Single-cell RNA-seq analysis reveals BHLHE40-driven pro-tumour neutrophils with hyperactivated glycolysis in pancreatic tumour microenvironment. Gut. 2023 May

2. Zhang Y et al. BHLHE40 promotes osteoclastogenesis and abnormal bone resorption via c-Fos/NFATc1. Cell Biosci. 2022 May

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: O14503 Human

Synonyms

BHE40_HUMAN antibody

bHLHB2 antibody

bHLHe40 antibody

Class B Basic Helix Loop Helix Protein 2 antibody

Class B basic helix-loop-helix protein 2 antibody

Class E basic helix loop helix protein 40 antibody

Class E basic helix-loop-helix protein 40 antibody

Clast5 antibody

DEC1 antibody

Differentially expressed in chondrocytes protein 1 antibody

Expand

Images

  • Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (<a href="/products/HA750764" style="font-weight: bold;text-decoration: underline;">HA750764</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: MDA-MB-231 cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: A431 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 46/50 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750764" style="font-weight: bold;text-decoration: underline;">HA750764</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (HA750764) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: MDA-MB-231 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: A431 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 46/50 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750764) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of MCF7 cells labeling SHARP2 with Rabbit anti-SHARP2 antibody (<a href="/products/HA750764" style="font-weight: bold;text-decoration: underline;">HA750764</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SHARP2 antibody (<a href="/products/HA750764" style="font-weight: bold;text-decoration: underline;">HA750764</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of MCF7 cells labeling SHARP2 with Rabbit anti-SHARP2 antibody (HA750764) at 1/100 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SHARP2 antibody (HA750764) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-SHARP2 antibody (<a href="/products/HA750764" style="font-weight: bold;text-decoration: underline;">HA750764</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750764" style="font-weight: bold;text-decoration: underline;">HA750764</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-SHARP2 antibody (HA750764) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750764) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"