RNF114 Recombinant Rabbit Monoclonal Antibody [PSH01-99] - BSA and Azide free
Catalog# HA750773
RNF114 Recombinant Rabbit Monoclonal Antibody [PSH01-99] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
RNF114 Recombinant Rabbit Monoclonal Antibody [PSH01-99] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RNF114 aa 1-228 / 228.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
SK-Br-3 cell lysate, Jurkat cell lysate.
Conjugation
unconjugated
Clone Number
PSH01-99
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
E3 ubiquitin-protein ligase RNF114 is a protein that in humans is encoded by the RNF114 gene. E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Acts also as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis.
Background References
1. Feng Z et al. RNF114 Silencing Inhibits the Proliferation and Metastasis of Gastric Cancer. J Cancer. 2022 Jan
2. Zhou S et al. Maternal RNF114-mediated target substrate degradation regulates zygotic genome activation in mouse embryos. Development. 2021 Jul
Subcellular Location
Cytoplasm, Nucleus.
UNIPROT
Synonyms
1110008J21Rik antibody
AI225886 antibody
AW549494 antibody
MGC112560 antibody
OTTMUSP00000001082 antibody
OTTMUSP00000017392 antibody
PSORS12 antibody
RING finger protein 114 antibody
RN114_HUMAN antibody
RNF114 antibody
ExpandImages
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Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA750773) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750773) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (HA750773) at 1/1,000 dilution.
Lane 1: 293T-si NT cell lysate
Lane 2: 293T-si RNF114 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"