Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-08] - BSA and Azide free
Catalog# HA750778
Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-08] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IP
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Human
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HA721767
含抗保成分
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unconjugated
Overview
Product Name
Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL-18 aa 37-193.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue, IP
Molecular Weight
Predicted band size: 22 kDa
Positive Control
U-2 OS cell lysate, A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, human liver tissue, human spleen tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
PSH02-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:5,000-1:10,000
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IF-Tissue
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1:1,000
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IP
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1-2μg/sample
Target
Function
Interleukin-18 (IL-18), also known as interferon-gamma inducing factor is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases. IL-18 belongs to the IL-1 superfamily and is produced mainly by macrophages but also other cell types, stimulates various cell types and has pleiotropic functions. IL-18 is a proinflammatory cytokine that facilitates type 1 responses. Together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 in combination with IL12 acts on CD4, CD8 T cells and NK cells to induce IFNγ production, type II interferon that plays an important role in activating the macrophages or other cells. The combination of this IL-18 and IL-12 has been shown to inhibit IL-4 dependent IgE and IgG1 production and enhance IgG2a production in B cells. Importantly, without IL-12 or IL-15, IL-18 does not induce IFNγ production, but plays an important role in the differentiation of naive T cells into Th2 cells and stimulates mast cells and basophils to produce IL-4, IL-13, and chemical mediators such as histamine.
Background References
1. Zhang X et al. IL18 signaling causes islet β cell development and insulin secretion via different receptors on acinar and β cells. Dev Cell. 2022 Jun
2. Lin T et al. NET-Triggered NLRP3 Activation and IL18 Release Drive Oxaliplatin-Induced Peripheral Neuropathy. Cancer Immunol Res. 2022 Dec
Subcellular Location
Cytoplasm, Secreted.
UNIPROT
Synonyms
Iboctadekin antibody
IFN gamma inducing factor antibody
IFN-gamma-inducing factor antibody
IGIF antibody
IL 1 gamma antibody
IL 18 antibody
IL 1g antibody
IL-1 gamma antibody
IL-18 antibody
IL1 gamma antibody
ExpandImages
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Western blot analysis of Human IL-18 on different lysates with Rabbit anti-Human IL-18 antibody (HA750778) at 1/1,000 dilution.
Lane 1: U-2 OS cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 1 minute 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750778) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Human IL-18 antibody (HA750778) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750778) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Human IL-18 antibody (HA750778) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750778) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Human IL-18 antibody (HA750778) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750778) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Human IL-18 was immunoprecipitated from 0.2 mg A549 cell lysate with HA750778 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750778 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (input)
Lane 2: Rabbit IgG instead of HA750778 in A549 cell lysate
Lane 3: HA750778 IP in A549 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 5 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-08]
Application: WB,IHC-P,IF-Tissue,IP
Reactivity: Human
Conjugate: unconjugated
Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-09] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
