Phospho-p70 S6 Kinase (T389) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH02-23] (HA750791)
Catalog# HA750791
Rabbit Monoclonal to Phospho-p70 S6 Kinase (T389)
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-p70 S6 Kinase (T389) on different lysates with Rabbit anti-Phospho-p70 S6 Kinase (T389) antibody (HA750791) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 70/85 kDa
Exposure time: Lane 1-4 (left): 1 minute 2 seconds; Lane 1-4 (right): 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750791) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-p70 S6 Kinase (T389) on different lysates with Rabbit anti-Phospho-p70 S6 Kinase (T389) antibody (HA750791) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 70/85 kDa
Exposure time: Lane 1-4 (left): 1 minute 2 seconds; Lane 1-4 (right): 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750791) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.