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SCP3 Recombinant Rabbit Monoclonal Antibody [PSH02-24] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750792

SCP3 Recombinant Rabbit Monoclonal Antibody [PSH02-24] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SCP3 Recombinant Rabbit Monoclonal Antibody [PSH02-24] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SCP3 aa 35-236 / 236 (Q8IZU3).

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 28 kDa

Positive Control

Human testis tissue.

Conjugation

unconjugated

Clone Number

PSH02-24

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells (By similarity). Required for normal meiosis during spermatogenesis and male fertility 1. Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2.

Background References

1. Oh SJ et al. Interaction between SCP3 and JAB1 Confers Cancer Therapeutic Resistance and Stem-like Properties through EGF Expression. Int J Mol Sci. 2021 Aug

2. Wang W et al. Expression of scp3 and dazl reveals the meiotic characteristics of the olive flounder Paralichthys olivaceus†. Biol Reprod. 2023 Feb

Subcellular Location

Nucleus, Chromosome, centromere.

UNIPROT

SwissProt: Q8IZU3 Human

Synonyms

choline phosphotransferase 1 antibody

chpt1 antibody

COR 1 antibody

COR1 antibody

MGC71888 antibody

RNASCP3 antibody

SCP 3 antibody

SCP-3 antibody

SCP3 antibody

SPGF4 antibody

Expand

Images

  • Western blot analysis of SCP3 on SCP3 recombinant protein with Rabbit anti-SCP3 antibody (<a href="/products/HA750792" style="font-weight: bold;text-decoration: underline;">HA750792</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 30 ng/Lane.<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750792" style="font-weight: bold;text-decoration: underline;">HA750792</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SCP3 on SCP3 recombinant protein with Rabbit anti-SCP3 antibody (HA750792) at 1/1,000 dilution.

    Lysates/proteins at 30 ng/Lane.

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750792) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SCP3 antibody (<a href="/products/HA750792" style="font-weight: bold;text-decoration: underline;">HA750792</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750792" style="font-weight: bold;text-decoration: underline;">HA750792</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SCP3 antibody (HA750792) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750792) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"