BAF180 Recombinant Rabbit Monoclonal Antibody [PSH02-34] - BSA and Azide free
Catalog# HA750799
BAF180 Recombinant Rabbit Monoclonal Antibody [PSH02-34] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
BAF180 Recombinant Rabbit Monoclonal Antibody [PSH02-34] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human BAF180 aa 1-300 / 1,689.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 193 kDa
Positive Control
293T cell lysate, HeLa cell lysate, SK-MEL-28 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, human breast tissue, HEK-293 cell lysate, MCF7 cell lysate, A549 cell lysate, Human kidney tissue lysate, mouse breast tissue, mouse kidney tissue, rat breast tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
PSH02-34
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.
Background References
1. Liu XD, Kong W, Peterson CB, et al. PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma. Nat Commun. 2020 May 1;11(1):2135.
2. De Silva SM, Dhiman A, Sood S, et al. PBRM1 bromodomains associate with RNA to facilitate chromatin association. Nucleic Acids Res. 2023 May 8;51(8):3631-3649.
Subcellular Location
Nucleus
UNIPROT
Synonyms
BAF180 antibody
BRG1-associated factor 180 antibody
CG11375 antibody
hPB1 antibody
MGC156155 antibody
MGC156156 antibody
OTTHUMP00000202149 antibody
OTTHUMP00000202150 antibody
OTTHUMP00000202151 antibody
OTTHUMP00000202153 antibody
ExpandImages
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This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Western blot analysis of BAF180 on different lysates with Rabbit anti-BAF180 antibody (HA721811) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721811) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAF180 antibody (HA721811) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Western blot analysis of BAF180 on different lysates with Rabbit anti-BAF180 antibody (HA721811) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: 293T cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: COS-1 cell lysate (20 µg/Lane)
Lane 10: Human kidney tissue lysate (40 µg/Lane)
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minute 41 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721811) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-BAF180 antibody (HA721811) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BAF180 antibody (HA721811) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-BAF180 antibody (HA721811) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BAF180 antibody (HA721811) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling BAF180 with Rabbit anti-BAF180 antibody (HA721811) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721811, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of paraffin-embedded mouse breast tissue labeling BAF180 with Rabbit anti-BAF180 antibody (HA721811) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721811, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Knockdown (KD)
This data was developed using HA721811, the same antibody clone in a different buffer formulation.
Western blot analysis of BAF180 on different lysates with Rabbit anti-BAF180 antibody (HA721811) at 1/2,000 dilution.
Lane 1: 293T-si NT cell lysate
Lane 2: 293T-si BAF180 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minute 22 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721811) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"