REIIBP/NSD2 Recombinant Rabbit Monoclonal Antibody [PSH02-39] - BSA and Azide free
Catalog# HA750804
REIIBP/NSD2 Recombinant Rabbit Monoclonal Antibody [PSH02-39] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA721816
含抗保成分
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unconjugated
Overview
Product Name
REIIBP/NSD2 Recombinant Rabbit Monoclonal Antibody [PSH02-39] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human NSD2 385-584 / 584 (O96028-4).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 66 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Rat testis tissue.
Conjugation
unconjugated
Clone Number
PSH02-39
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
Target
Function
NSD2 is a histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) . Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro . Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells. By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes. In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation. During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes. Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation. During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation. During B-cell development, required for the generation of the B1 lineage. During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response. Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation. By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA.
Background References
1. Tang W et al. METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathy. BMC Nephrol 23:124 (2022).
2. Want MY et al. WHSC1/NSD2 regulates immune infiltration in prostate cancer. J Immunother Cancer 9:N/A (2021).
Subcellular Location
Cytoplasm, nucleus, nucleolus
Synonyms
FLJ23286 antibody
IL5 promoter REII region binding protein antibody
KIAA1090 antibody
MGC176638 antibody
MMSET antibody
MMSET type II antibody
Multiple myeloma SET domain containing protein type III antibody
Multiple myeloma SET domain protein antibody
Multiple myeloma SET domain-containing protein antibody
NSD 2 antibody
ExpandImages
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Western blot analysis of REIIBP/NSD2 on different lysates with Rabbit anti-REIIBP/NSD2 antibody (HA750804) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Neuro-2a cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750804) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Rat testis tissue with Rabbit anti-REIIBP/NSD2 antibody (HA750804) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750804) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"