SLC25A38 Recombinant Rabbit Monoclonal Antibody [PSH02-79] - BSA and Azide free
Catalog# HA750834
SLC25A38 Recombinant Rabbit Monoclonal Antibody [PSH02-79] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
SLC25A38 Recombinant Rabbit Monoclonal Antibody [PSH02-79] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SLC25A38 aa 255-304 / 304.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
Human liver tissue, mouse liver tissue.
Conjugation
unconjugated
Clone Number
PSH02-79
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This gene is a member of the mitochondrial carrier family. The encoded protein is required during erythropoiesis and is important for the biosynthesis of heme. Mutations in this gene are the cause of autosomal congenital sideroblastic anemia (anemia, sideroblastic, 2, pyridoxine-refractory). A related pseudogene is found on chromosome 1. Mitochondrial glycine transporter is a protein that in humans is encoded by the SLC25A38 gene. SLC25A38 is involved in mitochondrial handling of glycine and is needed for the first step in heme synthesis. Mutations in this gene can lead to an autosomal recessive form of sideroblastic anemia.
Background References
1. Fan Z et al. SLC25A38 as a novel biomarker for metastasis and clinical outcome in uveal melanoma. Cell Death Dis. 2022 Apr
2. Ravindra N et al. Novel frameshift variant (c.409dupG) in SLC25A38 is a common cause of congenital sideroblastic anaemia in the Indian subcontinent. J Clin Pathol. 2021 Mar
Subcellular Location
Mitochondrion inner membrane.
Synonyms
FLJ20551 antibody
FLJ22703 antibody
S2538_HUMAN antibody
slc25a38 antibody
Solute carrier family 25 member 38 antibody
Images
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☑ Cell treatment (CT)
This data was developed using HA721857, the same antibody clone in a different buffer formulation.
Western blot analysis of SLC25A38 on different lysates with Rabbit anti-SLC25A38 antibody (HA721857) at 1/1,000 dilution.
Lane 1: 293T-NT cell lysate (no heat)
Lane 2: 293T-OE-SLC25A38 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721857) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
This data was developed using HA721857, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of 293T transfected with or without SLC25A38 cells labeling SLC25A38 with Rabbit anti-SLC25A38 antibody (HA721857) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLC25A38 antibody (HA721857) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using HA721857, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SLC25A38 antibody (HA721857) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721857) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721857, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SLC25A38 antibody (HA721857) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721857) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"