GALT Recombinant Rabbit Monoclonal Antibody [PSH02-87] - BSA and Azide free
Catalog# HA750841
GALT Recombinant Rabbit Monoclonal Antibody [PSH02-87] - BSA and Azide free
-
WB
-
IF-Cell
-
FC
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
GALT Recombinant Rabbit Monoclonal Antibody [PSH02-87] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human GALT aa 1-379 / 379.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, rat liver tissue lysate, HeLa, NIH/3T3.
Conjugation
unconjugated
Clone Number
PSH02-87
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Galactose-1-phosphate uridylyltransferase (or GALT, G1PUT) is an enzyme responsible for converting ingested galactose to glucose. Deficiency of GALT causes classic galactosemia. Galactosemia is an autosomal recessive inherited disorder detectable in newborns and childhood. Classical galactosemia (G/G) is caused by a deficiency in GALT activity, whereas the more common clinical manifestations, Duarte (D/D) and the Duarte/Classical variant (D/G) are caused by the attenuation of GALT activity. Symptoms include ovarian failure, developmental coordination disorder (difficulty speaking correctly and consistently), and neurologic deficits. A single mutation in any of several base pairs can lead to deficiency in GALT activity. Screening has mostly eliminated neonatal death by G/G galactosemia, but the disease, due to GALT’s role in the biochemical metabolism of ingested galactose (which is toxic when accumulated) to the energetically useful glucose, can certainly be fatal. However, those afflicted with galactosemia can live relatively normal lives by avoiding milk products and anything else containing galactose (because it cannot be metabolized), but there is still the potential for problems in neurological development or other complications, even in those who avoid galactose.
Background References
1. Latchman K. et. al. A founder noncoding GALT variant interfering with splicing causes galactosemia. J Inherit Metab Dis. 2020 Nov
2. Mörbe UM. et. al. Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunol. 2021 Jul
Subcellular Location
Cytosol, Golgi apparatus, cytoplasm.
Synonyms
Gal 1 P uridylyltransferase antibody
Gal-1-P uridylyltransferase antibody
Galactose 1 phosphate uridyl transferase antibody
Galactose 1 phosphate uridylyltransferase antibody
Galactose-1-phosphate uridylyltransferase antibody
GALT antibody
GALT_HUMAN antibody
UDP glucose hexose 1 phosphate uridylyltransferase antibody
UDP-glucose--hexose-1-phosphate uridylyltransferase antibody
Images
-
This data was developed using HA721875, the same antibody clone in a different buffer formulation.
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721875) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721875) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using HA721875, the same antibody clone in a different buffer formulation.
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721875) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-GALT KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721875) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721875, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa cells labeling GALT with Rabbit anti-GALT antibody (HA721875) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GALT antibody (HA721875) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
This data was developed using HA721875, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NIH/3T3 cells labeling GALT with Rabbit anti-GALT antibody (HA721875) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GALT antibody (HA721875) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
This data was developed using HA721875, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HeLa cells labeling GALT.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721875, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
This data was developed using HA721875, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of NIH/3T3 cells labeling GALT.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721875, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"