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PPP2R1B Recombinant Rabbit Monoclonal Antibody [PSH02-92] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750847

PPP2R1B Recombinant Rabbit Monoclonal Antibody [PSH02-92] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PPP2R1B Recombinant Rabbit Monoclonal Antibody [PSH02-92] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PPP2R1B aa 1-601 / 601.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 66 kDa

Positive Control

Caco-2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human liver tissue, mouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH02-92

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

Background References

1. Abraham D, Podar K, Pacher M, Kubicek M, Welzel N, Hemmings BA, Dilworth SM, Mischak H, Kolch W, Baccarini M. Raf-1-associated protein phosphatase 2A as a positive regulator of kinase activation. J Biol Chem. 2000 Jul 21;275(29):22300-4.

2. Lubert EJ, Sarge KD. Interaction between protein phosphatase 2A and members of the importin beta superfamily. Biochem Biophys Res Commun. 2003 Apr 11;303(3):908-13.

3. Kitajima TS, Sakuno T, Ishiguro K, Iemura S, Natsume T, Kawashima SA, Watanabe Y. Shugoshin collaborates with protein phosphatase 2A to protect cohesin. Nature. 2006 May 4;441(7089):46-52.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P30154 Human

SwissProt: Q7TNP2 Mouse

SwissProt: Q4QQT4 Rat

Synonyms

2AAB_HUMAN antibody

beta isoform of regulatory subunit A, protein phosphatase 2 antibody

PP2A A beta antibody

PP2A subunit A isoform PR65-beta antibody

PP2A subunit A isoform R1-beta antibody

PP2A, subunit A, PR65 beta isoform antibody

PP2A, subunit A, R1 beta isoform antibody

PPP2R1B antibody

PPP4R1 antibody

PR65B antibody

Expand

Images

  • Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: Mouse testis tissue lysate<br />Lane 7: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA750847) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: Mouse testis tissue lysate
    Lane 7: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750847) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PPP2R1B antibody (HA750847) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750847) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PPP2R1B antibody (HA750847) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PPP2R1B antibody (HA750847) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PPP2R1B antibody (HA750847) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750847) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PPP2R1B antibody (HA750847) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate (10 µg/Lane)<br />Lane 2: HeLa-si PPP2R1B cell lysate (10 µg/Lane)<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 16 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750847" style="font-weight: bold;text-decoration: underline;">HA750847</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA750847) at 1/2,000 dilution.

    Lane 1: HeLa-si NT cell lysate (10 µg/Lane)
    Lane 2: HeLa-si PPP2R1B cell lysate (10 µg/Lane)

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 16 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750847) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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