Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] - BSA and Azide free
Catalog# HA750855
Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] - BSA and Azide free
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Axin1 aa 1-400 / 862.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 95.6 kDa
Positive Control
SW480 cell lysate, HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Huh7 cell lysate, human colon cancer tissue.
Conjugation
unconjugated
Clone Number
PSH03-00
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin . Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling . In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) . Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice . Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop .
Background References
1. Luo W, Lin SC. Axin: a master scaffold for multiple signaling pathways. Neurosignals. 2004 May-Jun;13(3):99-113.
2. Salahshor S, Woodgett JR. The links between axin and carcinogenesis. J Clin Pathol. 2005 Mar;58(3):225-36.
3. Mallick A, Gupta BP. AXIN-AMPK signaling: Implications for healthy aging. F1000Res. 2021 Dec 8;10:1259.
Subcellular Location
Cytoplasm. Nucleus. Membrane. Cell membrane.
UNIPROT
Synonyms
AI316800 antibody
AXIN antibody
Axin 1 antibody
Axin-1 antibody
axin1 antibody
AXIN1_HUMAN antibody
Axis inhibition protein 1 antibody
Axis Inhibitor 1 antibody
Fu antibody
fused antibody
ExpandImages
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This data was developed using HA721919, the same antibody clone in a different buffer formulation.
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution.
Lane 1: SW480 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: 293T cell lysate (10 µg/Lane)
Lane 4: K-562 cell lysate (10 µg/Lane)
Lane 5: Huh7 cell lysate (10 µg/Lane)
Predicted band size: 96 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using HA721919, the same antibody clone in a different buffer formulation.
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Axin1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 110 kDa
Exposure time: 140 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721919, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721919) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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