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Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750855

Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Axin1 aa 1-400 / 862.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 95.6 kDa

Positive Control

SW480 cell lysate, HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Huh7 cell lysate, human colon cancer tissue.

Conjugation

unconjugated

Clone Number

PSH03-00

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500-1:2,000

Target

Function

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin . Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling . In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) . Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice . Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop .

Background References

1. Luo W, Lin SC. Axin: a master scaffold for multiple signaling pathways. Neurosignals. 2004 May-Jun;13(3):99-113.

2. Salahshor S, Woodgett JR. The links between axin and carcinogenesis. J Clin Pathol. 2005 Mar;58(3):225-36.

3. Mallick A, Gupta BP. AXIN-AMPK signaling: Implications for healthy aging. F1000Res. 2021 Dec 8;10:1259.

Subcellular Location

Cytoplasm. Nucleus. Membrane. Cell membrane.

UNIPROT

SwissProt: O15169 Human

Synonyms

AI316800 antibody

AXIN antibody

Axin 1 antibody

Axin-1 antibody

axin1 antibody

AXIN1_HUMAN antibody

Axis inhibition protein 1 antibody

Axis Inhibitor 1 antibody

Fu antibody

fused antibody

Expand

Images

  • Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (<a href="/products/HA750855" style="font-weight: bold;text-decoration: underline;">HA750855</a>) at 1/2,000 dilution.<br /><br />Lane 1: SW480 cell lysate (10 µg/Lane)<br />Lane 2: HeLa cell lysate (10 µg/Lane)<br />Lane 3: 293T cell lysate (10 µg/Lane)<br />Lane 4: K-562 cell lysate (10 µg/Lane)<br />Lane 5: Huh7 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 96 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750855" style="font-weight: bold;text-decoration: underline;">HA750855</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA750855) at 1/2,000 dilution.

    Lane 1: SW480 cell lysate (10 µg/Lane)
    Lane 2: HeLa cell lysate (10 µg/Lane)
    Lane 3: 293T cell lysate (10 µg/Lane)
    Lane 4: K-562 cell lysate (10 µg/Lane)
    Lane 5: Huh7 cell lysate (10 µg/Lane)

    Predicted band size: 96 kDa
    Observed band size: 110 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750855) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (<a href="/products/HA750855" style="font-weight: bold;text-decoration: underline;">HA750855</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Axin1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 140 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750855" style="font-weight: bold;text-decoration: underline;">HA750855</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA750855) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Axin1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 110 kDa

    Exposure time: 140 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750855) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin1 antibody (<a href="/products/HA750855" style="font-weight: bold;text-decoration: underline;">HA750855</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750855" style="font-weight: bold;text-decoration: underline;">HA750855</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin1 antibody (HA750855) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750855) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00]

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated